Session: Symposium: Emerging and Reemerging Infections
Monday, October 27, 2008: 12:00 AM
Room: Room 202B
A 57-year old male patient died of an intracerebral hemorrhage ten days after returning from a trip to rural Yugoslavia. His kidneys and liver were donated to three female recipients aged 44 years (kidney), 63 years (kidney), and 64 years (liver). Four to five weeks after organ donation, all three recipients died. All had febrile illnesses with altered mental status. Subsequent testing of post-mortem tissues from the recipients identified a new arenavirus, which was related to lymphocyctic choriomeningitis virus. This viral detection process involved the use of high-throughput sequencing techniques to identify novel microbial RNA sequences. Confirmatory testing was performed using the techniques of immunohistochemical analysis for arenavirus antigens in tissue specimens and immunofluorescent testing for IgG and IgM antibodies in serum samples. The lecture will discuss the clinical features in the four patients, details of the laboratory identification of this new virus, and implications for organ transplantation in future.