M-722. Molecular Typing of Yeast Strains Isolated from Symptomatic, Asymptomatic Women and Asymptomatic Pregnant Women in Ekpoma, Nigeria
Session: Poster Session: Fungal Epidemiology
Saturday, October 25, 2008: 12:00 AM
Room: Hall C
Background: The objective of the study was to characterize yeast strains from symptomatic, asymptomatic women and asymptomatic pregnant women.
Methods: In this study we collected 300 samples of high vaginal swab from symptomatic, asymptomatic women and asymptomatic pregnant women 100 each from each group. The swabs were cultured on SDA, CHROMagar Candida, carbohydrate assimilation was done using ID32C (Biomerieux, France) , PCR was done in all the isolate, MIC for Fluconazole and Itraconazole using E- test strip, growth at 450and in 6.5% Nacl was also evaluated. Results: From the study it was observed that there was no significant difference between the three groups studied with p- value > 0.05 and the most isolated species was Candida albicans 53%, Candida glabrata 20.5 %, Candida tropicalis 12.8%, Candida parapsilopsis 8.5% and others 5.1%. Co- habitation was also observed among the species. Fluconazole: MIC<8µg/ml was taken as sensitive; MIC>8µg/ml and ≤32µg/ml was considered sensitive but dose dependent (SDD) and MIC>32µg/ml was read as resistant. For Itraconazole: MIC ≤ 0.125µg/ml taken as sensitive; MIC> 0.125µg/ml and ≤ 0.5 µg/ml taken as sensitive but dose dependent and MIC > 0.5 µg/ml was read as resistant (NCCLS, 1997).
Conclusions: We found out that asymptomatic women harbor different Candida strains and that there was co - habitation within the same individual. It was also concluded that symptomatic vaginal Candidiasis may result from patient change in immune status which will allow the proliferation of the Candida strains that was kept in check.
Agwu Ezera, Bmls, MSc1, F Esumeh, BSc, MSc, PhD2, Helen Obiazi, BSc, MSc2, Ifeoma Enweani, BSc, Aimls, MSc, PhD3, Nyoho Inyang, Aimls, MSc4, Ruben Efedeyi, MBBS, FWACS5 and  N. J. Inyang, None., (1)Kampala international. university, (2)Ambrose Alli university, (3)Department of medical laboratory sciences, faculty of health sciences and technology, Nnamdi Azikewe university, (4)Specialist teaching hospital irrua, Irrua, Nigeria, (5)Specialist teaching hospital

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