D-1145. Comparison of Two PCR-Based Methods and Conventional Culture for the Detection of Nasal Carriage of Staphylococcus aureus in Preoperative Patients
Session: Poster Session: Staphylococcus aureus: Screening, Typing and Epidemiology
Sunday, October 26, 2008: 12:00 AM
Room: Hall C
Background: Nasal carriage is an important risk factor for the development of post-operative infections with S. aureus and pre-operative treatment with mupirocin in carriers reduces the post-operative infection rate. Therefore it is important to identify nasal carriage rapidly. Two PCR techniques were compared to conventional culture in surgical patients. Methods: In 404 consecutive patients nasal swabs were taken for preoperative screening for nasal carriage of S. aureus. The performance of the Roche Staphylococcus Kit on Lightcycler (Roche; RSA) and the Becton Dickinson (Bedford, MA) GeneOhm StaphSR assay on Smartcycler (Cepheid; BDSA) were compared with semi-quantitative culture. Results: Sensitivity for culture, RSA and BDSA compared to golden standard was 98.2 %, 82.0 % and 85.6 % respectively and specificity was 100 %, 98.3 % and 99.3 %, respectively. The lower sensitivity of both PCR techniques was associated with samples with low bacterial loads.Conclusions: The RSA and BDSA were similar in performance and are suitable for pre-operative identification of nasal carriers of S. aureus.
Annneke Bergmans1, Diana Bogaers1, Gunnar Andriesse, MD, PhD2, Jan Kluytmans, MD, Prof, PhD1, Miranda van Rijen2 and  G. I. Andriesse,
Becton Dickinson Received: Research Support., (1)Amphia Hospital, (2)Amphia Hospital, Breda, Netherlands