L-1500. Prevalence of Panton-Valentine Leukocidin in Staphylococcus aureus Isolated from Conjunctivitis or Blepharitis in the United States and India
Session: Poster Session: Community-Acquired MRSA
Sunday, October 26, 2008: 12:00 AM
Room: Hall C
Staphylococcus aureus Panton-Valentine Leukocidin (PVL) has been reported to be associated with community-acquired methicillin-resistance, predominately in skin and soft tissue infections and necrotizing pneumonia. S. aureus is a major etiologic agent of blepharitis and conjunctivitis. This study was conducted to determine prevalence of PVL and methicillin-resistance in S. aureus recovered between 2006-2007 from conjunctivitis or blepharitis patients in the United States and India. Methods:
S. aureus were identified by automated ribotyping (RiboPrinter, Dupont). Detection of the genes encoding PVL (lukS-PV and lukF-PV) was performed by PCR. Antibiotic susceptibility profiles were determined by microtiter broth dilution (CLSI).
PVL was detected more frequently in S. aureus ocular isolates from India (35%) than the United States (9%). In India, 80% (21 of 25) of the PVL-positive isolates were methicillin (oxacillin)-susceptible. In contrast, of the 6 PVL-positive isolates from the US, only 33% (2 of 6) were methicillin-susceptible.
DiseaseCountry of OriginNo. of IsolatesMethicillin-ResistantPVL- PositiveMethicillin- Susceptible and PVL-PositiveMethicillin- Resistant and PVL-Positive
ConjunctivitisUSA356 (17%)5 (14%)2 (40%)3 (60%)
India578 (14%)18 (32%)14 (78%)4 (22%)
BlepharitisUSA314 (13%)1 (3%)0 (0%)1 (100%)
India140 (0%)7 (50%)7 (100%)0 (0%)

In recent clinical studies, S. aureus isolates recovered from ocular infections in India were noteworthy in that PVL genes were not generally detected in methicillin-resistant isolates. This is in contrast to S. aureus isolates recovered from skin and soft tissue infections in many parts of the world.
David Stroman, PhD1, Joseph Dajcs2, Linda Clark, BS1 and  J. J. Dajcs, None., (1)Alcon Laboratories, Inc., (2)Alcon Laboratories, Inc., Fort Worth, TX

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