1731a. Persistence at the Single Cell Level
Session: Symposium: Antimicrobial Tolerance: Persisters, Biofilms, and Infectious Diseases
Sunday, October 26, 2008: 12:00 AM
Room: Room 144A
Phenotypic variability in populations of cells has been linked to evolutionary robustness to stressful conditions. Recently, sub-populations of bacteria, termed persisters, were shown to be responsible for the persistence of the population to antibiotic treatments. Direct observations of single persisters bacteria in microfluidic devices, with and without antibiotic treatment, are crucial for understanding the dynamics of persister formation. Here we use fluorescence induction of synthetic promoters in microfluidic devices to follow the differentiation into the dormant state of single persister bacteria. Our results show the power of single-cell induction dynamics in microfluidic devices for revealing subtle phenotypes in heterogeneous populations. Similar approach might shed light on the dormancy of sub-populations in different systems such as viable but non-culturable cells, or quiescent cancer cells.
Nathalie Balaban, PhD, Hebrew Univ. of Jerusalem, Jerusalem, Israel and  N. Q. Balaban, None.

NATHALIE
QUESTEMBERT-BALABAN
, Senior Lecturer in Physics
Born: Paris,
France. Ph.D.: Semi-conductors physics, Weizmann Institute,
1999.
Hebrew University: Senior Lecturer, 2003.
Other
Appointments
: Post-doctoral Fellow, Rockefeller University, 2001-2003; Dicke Fellow;
Princeton University, 2000-2001. Awards
and honors
: Feinberg Graduate Shool Award for Excellence in Ms.Sc. Studies,
1992;Dicke
Fellowship, 2000; Horowitz Foundation Fellow, 2004.
Research Interests: Biological Physics; Quantitative
measurements of biological systems; Development of soft lithography and micro-fluidic
tools for controlled experiments on single cells; Mathematical modeling of
biological variability.
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