558. Biofilm Formation on Standard and Novel, Silver Impregnated Intravenous Catheter Access Devices
Session: Poster Session: Hospital-acquired and Transplant Infections
Friday, October 30, 2009: 12:00 AM
Room: Poster Hall A
Background: Catheter Access Devices (CADs) can be colonized by biofilms, an important factor for developing catheter-associated bloodstream infections. The objective of this study was to determine the incidence and burden of biofilm formation on 2 types of CADs obtained from critically ill patients: a standard Luer-activated CAD with a valve, and an identically designed CAD with a novel, silver intraluminal coating.
Methods: CADs were obtained from patients eligible for routine change of these devices in the intensive care units at 2 university hospitals. As part of routine patient care, CADs are changed weekly on central lines. Protocols were developed for collection, shipment, and processing of CADs in order to minimize contamination and maximize recovery of biofilm organisms. Biofilms were quantified by growth of microorganisms recovered from CAD luminal surfaces on microbiological media.
Results: From 6 patients, 11 standard and 3 novel CADs were collected from peripherally inserted central venous catheters (43%) and central lines (57%). Most catheters (80%) had 2 or 3 lumens. Biofilms were detected in 9/14 CADs (64%), with between 5 and 107 colony-forming units (CFU) per device. Four CADs had counts >103 CFU. Among the standard CADs, 8/11 (73%) had detectable biofilm. Of the novel CADs, 1/3 (33%) contained biofilm; the CAD with biofilm had 5 CFU, a value at the threshold of detection. Of the 5 patients who had CADs collected from different lumens of the same catheter, 3 had biofilm counts that differed by 103 to 105 CFU. The other 2 patients had ≤5 CFU on all CADs.
Conclusion: Biofilms were detected in 64% of CADs and several contained more than 103 CFU per device. Silver impregnated CADs were less prone to biofilm formation, but the small sample size precluded comparison to the standard devices. There was substantial variability in biofilm formation on CADs, even among devices obtained from the same catheter.
Rodney Donlan, PhD, Centers for Disease Control and Prevention, Atlanta,, GA, Jesse Jacob, MD, Emory University, Atlanta, GA, Elizabeth Perez, BS, Centers for Disease Control and Prevention, Atlanta, GA, James Steinberg, MD and  R. M. Donlan, None..
E. Perez, None. 
J. P. Steinberg,
Baxter Healthcare Corp Received: Research Grant.
J. T. Jacob,
Baxter Healthcare Corp Received: Research Grant.