688. Detection of Human Papillomavirus in Vaginal Lesions Using in Situ Hybridization
Session: Abstracts: Virology
Friday, October 22, 2010
Background: Data on human papillomavirus (HPV) prevalence in vaginal cancers are limited. These data are important to predict the potential future effect of prophylactic HPV vaccines. Polymerase chain reaction (PCR) and in situ hybridization (ISH) have increasingly been used for detection of HPV in cervicovaginal samples. However, there are limited studies in the literature regarding detection of HPV in vaginal lesions using ISH.

Methods: Vaginal biopsies from 26 female patients were studied in which presence of the high and low risk subtypes of HPV virus was determined by in situ hybridization (ISH) using commercial HPV probes.

Results: The diagnoses were as follows: 12 (46.2 %) negative biopsies, 6 (23.1%) condylomas without overt dysplasia, 4 (15.4%) vaginal intraepithelial neoplasia I (VAIN I), 2 (7.7%) VAIN II, 1 (3.8%) VAIN III and 1 carcinoma (3.8%). Low risk HPV was detected in 2/12 (16.7%) of negative cases, 4/6 (66.7%) of condylomas, 1/4 (25%) VAIN I. High risk HPV was detected in 1/4 (25%) cases of VAIN I, in 1/2 (50%) cases of VAIN II and in the one case of vaginal carcinoma. HPV cytopathic effect was noticed in 16 cases (61.5%) and more specifically in 9 (75%) negative biopsies which included 4 cases of squamous papillomas, 4 (66.7%) condylomas, 2 (50%) cases of VAIN I and 1 (50%) case of VAIN II.

Conclusion: We conclude that when used together and evaluated in conjunction with histology sections, ISH is a useful tool for ancillary molecular testing of HPV infection in vaginal lesions. When VAIN, condylomas or histological markers of HPV infection in vaginal lesions are detected, ISH can be used for detection of HPV in these tissues.

Subject Category: V. Virology including clinical and basic studies of viral infections, including hepatitis

Theodoros Kelesidis, MD , Ronald Reagan UCLA Medical Center, Los Angeles, CA
Petros Karakitsos, MD , Cytopathology, University of Athens School of Medicine, Athens, Greece
Sotirios Tsiodras, MD , 4th Dpt of Internal Medicine, University of Athens Medical School, Athens, Greece


T. Kelesidis, None

P. Karakitsos, None

S. Tsiodras, None

See more of: Virology
See more of: Abstracts

Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 21 with the exception of research findings presented at IDSA press conferences.


Copyright IDSA 2009 Infectious Diseases Society of America 1300 Wilson Boulevard, Suite 300 Arlington, VA 22209 info@idsociety.org