693. Expression Coxsackievirus Adenovirus Receptor (CAR) in Myocardial Tissues of Dilated Cardiomyopathy Cases
Session: Abstracts: Virology
Friday, October 22, 2010
Background:

Myocarditis and dilated cardiomyopathy (DCM) are common causes of morbidity and mortality in children and adults. Recently, the human coxsackievirus and adenovirus receptor (HCAR), a common receptor for the most common viral causes of myocarditis (coxsackieviruses B3 & B4 and adenoviruses 2 & 5), has been discovered. The present study has been designed therefore to study the expression of HCAR and detection of adenoviral genome in autopsied myocardial tissue of patients with DCM.

Methods: Formalin fixed myocardial tissues collected during autopsy from 26 cases of DCM and 20 cases each of  non-cardiac disease and cardiac disease other than DCM were included as test group, control group-A and B respectively. Expression of CAR was studied by immunohistochemical staining of myocardial tissue using CAR specific rabbit polyclonal antibody. The tissue sections were considered positive when >25% of the cell showed brown color staining. Detection of adenovirus DNA was done by polymerase chain reaction using group specific primers.

Results:

The CAR positivity in DCM cases was found to be 96% (25/26) as compared to 30% in control group A and 40% in control group B respectively. CAR was found to be expressed in myocytes, endothelial and interstitial cells, however positivity in myocytes was significantly higher than other cells (P=0.0006 & P=0.0001). No significant difference was observed in CAR positivity between endothelial and interstitial cells. The site of CAR expression was predominantly in the sarcolemma along with cytoplasm in cardiomyocytes. Adenovirus DNA was detected in myocardial tissue of one DCM case and none in control groups.

Conclusion:

The present study has highlighted the higher expression of CAR in DCM cases and significance of CAR expression in myocyte and endothelial cells.  The viral receptor based pathogenesis would help in appropriate diagnostic and therapeutic approach in management of viral myocarditis and DCM cases.


Subject Category: V. Virology including clinical and basic studies of viral infections, including hepatitis

Speakers:
Baijayantimala Mishra, MD , Department of Virology, Post Graduate Institute of Medical Education & Research,, Chandigarh, India
Tripta Kaur, MD , Department of Virology, Post Graduate Institute of Medical Education & Research,, Chandigarh, India
Uma Nahar Saikia, MD , Department of Histopathology, Post Graduate Institute of Medical Education & Research,, Chandigarh, India
Ajay Bahl, MD, DM , Department of Cardiology, Post Graduate Institute of Medical Education & Research,, Chandigarh, India
Radha Kanta Ratho, MD , Department of Virology, Post Graduate Institute of Medical Education & Research,, Chandigarh, India

Disclosures:

B. Mishra, None

T. Kaur, None

U. N. Saikia, None

A. Bahl, None

R. K. Ratho, None

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