698. Presence of Andes Virus Genome in Gingival Crevicular Fluid During Acute Hantavirus Infection
Session: Abstracts: Virology
Friday, October 22, 2010
Background: NIH grant # U19 AI 45452 Person to person transmission of Andes virus (ANDV) is well documented in Argentina and Chile. An increased risk of transmission of ANDV has been evident among sexual partners and others with close contact compared with other household members who did not have sexual or other close contact with index cases. The finding of ANDV in body fluids could help to advance our understanding of the risk factors for transmission of ANDV in the household setting.

We searched for the live virus and genomic ANDV RNA in saliva, gingival crevicular fluid (GCF), respiratory secretions (RS), blood, urine, and genital fluids obtained during the acute phase of the disease among index cases.  Simultaneously, we prospectively followed their close contacts for five weeks (one incubation period) looking for evidence of viral RNA in the same body secretions before the appearance of anti-ANDV antibodies.

Methods: We used real time RT-PCR for ANDV with S-genomic primers using blood cells, GCF, saliva, RS, urine and genital secretions as template. Viral culture in Vero E6 cells from PCR-positive samples was attempted through 3 blind passages (6 weeks)

Results: Nineteen confirmed hantavirus index cases have been examined, 10 (53%) men, 8 (42%) developed severe disease, and 4 (21%) died, 25 contacts were enrolled (10 sexual partners, 8 children, 3 mothers and 4 other relatives). In one couple we diagnosed ANDV infection in both, in fact the “index case” turned out to be second case in the cluster. None of the other enrolled household contacts have developed the disease and their ANDV virology test have been negative during the follow up period. To date, we have detected ANDV RNA in all the index cases’ blood cells, in 8/16 plasma samples, in 3/11 (27%) RS, in 1/15 urines, in 6/14 (43%) GCF samples; in one of them we were also able to detect ANDV RNA after 3 passages in Vero E6. In contrast, we have been able to detect ANDV RNA from saliva in 1/12 index patients.

Conclusion: Detection of ANDV RNA in GCF samples from patients with HCPS suggests a potential role for a periodontal viral pool in transmission and might lead to new prophylactic approaches toward preventing intrafamilial transmission. Further investigations will concentrate on detection of infectious virus in these samples.

Subject Category: V. Virology including clinical and basic studies of viral infections, including hepatitis

Marcela Ferres, MD, MPH , Pediatrics, Pontificia Universidad Católica de Chile, Santiago, Chile
Pablo Vial, MD , Pediatrics, University Del Desarrolo, Santiago, Chile
Claudia Marco, RN , Universidad del Desarrollo, Santiago, Chile
Constanza Martinez, Biochemist , Virology Laboratory, Pontificia Universidad Catolica de Chile, Santiago, Chile
Paula Godoy, Biochemist , Virology Laboratory, Pontificia Universidad Catolica de Chile, Santiago, Chile
Analia Cuiza, RN , Universidad del Desarrollo, Santiago, Chile
Francisca Valdivieso, MD , Universidad del Desarrollo, Santiago, Chile
Brian Hjelle, MD , Pathology, University of New Mexico, Albuquerque, NM
Gregory Mertz, MD , Internal Medicine, University of New Mexico School of Medicine, Albuquerque, NM


M. Ferres, None

P. Vial, None

C. Marco, None

C. Martinez, None

P. Godoy, None

A. Cuiza, None

F. Valdivieso, None

B. Hjelle, None

G. Mertz, None

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