1144. The changes and its significance of in peripheral blood of the patients with multidrug-resistant and extensively drug-resistant pulmonary tuberculosis
Session: Poster Abstract Session: Innate and Adaptive Immunity to Infections
Saturday, October 22, 2011
Room: Poster Hall B1
Background: China is one of the world’s 22 countries with the highest burden of tuberculosis. China is also one of the world’s 27 countries with the highest burden of MDR- TB/ XDR-TB.To explore the expression state and its significance of NKT cells, NK cells and T lymphocyte subsets in peripheral blood of the multi-drug resistant (MDR-TB) and extensively drug-resistant (XDR-TB) pulmonary tuberculosis patients.

Methods:Data of 316 patients with pulmonary tuberculosis hospitalized in Shanghai Pulmonary Hospital from January 2008 to June 2009 were retrospectively analyzed. According to the drug-resistance, these patients were divided into MDR group, XDR group and sensitive group. 146 patients belonged to MDR group. 77 patients belonged to XDR group. 93 patients belonged to susceptible group. According to the Range of cavitation in lung field area, these patients were also divided into 1-2 lung fields affected group with 132 patients, 3-4 lung fields affected group with 49 patients and 5-6 lung fields affected group with 9 patients. The frequencies of NKT cells, NK cells and T cells from whole blood were tested by flow cytometry.

 Results: The expression rate of NKT cells and NK cells was the highest 11(6,16)% and 7(4,12)% in the XDR group, MDR group was the second 8(5,14)% and 6(4,11)%, and sensitive group was the lowest 7(4,11)% and 5(3,9)%, among the three groups the difference was statistically significant (P<0.05). The expression rate of the NKT and NK cells in male of XDR group was significantly higher than that in female, CD4 and CD4/CD8 significantly lower than that of female, the difference was statistically significant (P<0.05, P<0.01). The expression rate of CD4 (42±9)% is the highest in the 1-2 lung fields,CD8 (22±8)% is the lowest; In the 5-6 lung fields, CD4 (36±11)% is the lowest, CD8 (28±12)% is the highest, CD4/CD8 (1.5±0.8) is the lowest, the difference was statistically significant ,P<0.01).

Conclusion: With the increasing level of drug resistance, the expression rate of NKT cells and NK cells increased, while the resistance and its level of tuberculosis patients did not affect the expression of T cells. The wider of the Range of cavitation in tuberculosis patients, the value of CD4 and CD4/CD8 in peripheral blood were lower, and CD8 was higher.


Subject Category: E. Innate and adaptive immunity to infections, including vaccine immunology

Shenjie Tang, Yan Yang, Qing Zhang, Hua Sun, Yidian Liu, Xiaohui Hao, Lan Yao and Jin Gu, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China

Disclosures:

S. Tang, None

Y. Yang, None

Q. Zhang, None

H. Sun, None

Y. Liu, None

X. Hao, None

L. Yao, None

J. Gu, None

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