456. Partial and near full length genome characterization of HIV-1 strains from recently infected individuals in São Paulo, Brazil
Session: Poster Abstract Session: HIV Pathogenesis and Immunity
Friday, October 21, 2011
Room: Poster Hall B1
Handouts
  • 456. Partial and near full length genome characterization of HIV-1 strains from recently infected individuals in São Paulo, Brazil.PNG (348.3 kB)
  • Background: 

    Here, we report partial and near full length genomic (NFLG) variability of HIV-1 isolates from well-characterized cohort of recently infected patients.

    Methods: HIV-1 proviral DNA was extracted from PMNCs of 113 participants. The NFLG were determined by overlapping nested PCR and direct sequencing. The data were phylogenetically inferred.

    Results: 

    Of the 113 samples, amplification and sequencing of NFLG was successful for 76 (67.3%) samples, of which 64 (84.2%) and 12 (15.8%) were pure and recombinants subtypes, respectively. Of the NFLG sequences, 62 (81.5%) of the non-recombinant isolates belonged to subtype B, one (1.3%) to sub-subtype F1 and subtype C each. All the 12 (15.9%) NFLG intersubtype mosaic isolates were unique recombinant forms and consisted of ten (13.1%) and two (2.6%) BF1 and BC variants, respectively. Partial amplification and sequencing of at least one of the five fragments was successful in 33 samples (29.2%) and only 4 samples (3.5%) could not amplify for any fragment. The distribution of genotypes among partial sequences was as follows: subtype B (25/33), subtype C and BF1 2/33 each and subtype F1, BC and CRF02_AG (1/33) each. The combined analysis of partial and NFLGs indicated that subtype B accounts for 79.8% followed by unique BF recombinants which represents 11% of the isolated variants.

    Conclusion: Our results confirmed the existence of various HIV subtypes currently circulating in Brazil and proved that subtype B is still the single most prevalent genetic form and responsible of 79.9% of the current HIV infection in São Paulo. Our data from partial and NFLGs showed that up to 20.2% of the samples from people whose HIV infection was newly diagnosed in the state of São Paulo belonged to non-B subtypes. Thereby, our data constitutes an important argument in favour of future design of multiepitope HIV vaccine suitable for the Brazilian population.


    Subject Category: H. HIV/AIDS and other retroviruses

    Antonio Charlys da Costa, MSc.1, Walter Kleine-Neto1, Evelin Regina Pastena2, Vanessa Pouza Martinez2, Mariana Melillo Sauer2, Helena Tomiyama2, Solange Oliveira2, Katia Bassichetto2, Ester Sabino2, Esper Kallas2 and Sabri Saeed Sanabani1, (1)Translational Medicine Department - Federal University of Sao Paulo, Sao Paulo, Brazil, (2)University of Sao Paulo, School of Medicine, Sao Paulo, Brazil

    Disclosures:

    A. C. D. Costa, None

    W. Kleine-Neto, None

    E. R. Pastena, None

    V. P. Martinez, None

    M. M. Sauer, None

    H. Tomiyama, None

    S. Oliveira, None

    K. Bassichetto, None

    E. Sabino, None

    E. Kallas, None

    S. S. Sanabani, None

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