1011. Comparison of the Ibis System Versus Traditional Clinical Microbiology for the Detection of Organisms Colonizing High use Surfaces in a Burn Unit and an Orthopaedic Ward
Session: Poster Abstract Session: Detecting, Identifying, and Typing Bacteria
Saturday, October 22, 2011
Room: Poster Hall B1
Background: Nosocomial transmission through fomites is an ongoing concern for the prevention of infection. Use of alternative methods, including molecular identification may aid targeted efforts to detect pathogens in the hospital environment.

Methods: We compared environmental contamination of high use sites between a burn unit (9 patient rooms) and an orthopaedic unit (10 patient rooms and 10 shears) using traditional clinical microbiology (TM) and the Ibis T5000TM Biosensor system.

Results:  Within the burn unit, clinically relevant gram negative bacteria were detected on 4 sites by TM and 21 by Ibis. S. aureus was detected on 5 sites by TM and 10 by Ibis. There were no clinically relevant gram negative bacteria recovered by TM in the orthopaedic wards while Ibis detected 19 gram negative bacteria. S. aureus was recovered in 3 (TM) and 4 (Ibis) sites on the orthopaedic ward.

 

Burn unit

positive sites (# isolates)

Ortho ward

positive sites(# isolates)

 

Screened

TM

Ibis

Screened

TM

Ibis

Bedrails

9

7(11)

9(44)

10

8(11)

10(84)

Door handle

9

6(7)

9(34)

10

3(3)

10(48)

Sink

9

7(8)

9(41)

10

9(11)

10(109)

IV pump

9

6(7)

8(34)

10

5(6)

10(50)

Keyboard

9

9(16)

9(48)

8

8(11)

8(35)

Mouse

9

6(8)

9(38)

6

4(7)

6(35)

Shear

0

 

 

10

7(8)

10(48)

Conclusion: The Ibis system was able to detect more pathogens including potentially virulent and more resistant pathogens, than standard clinical microbiology in both the burn unit and orthopaedic ward with relatively similar contamination rates between the units.  


Subject Category: N. Hospital-acquired and surgical infections, infection control, and health outcomes including general public health and health services research

Heather Yun, MD1, Helen Crouch, RN, MPH, CIC1, Bernadette Thompson, RN, BSN, CIC1, Myra Castillo, RN, BS, CIC2, Kevin K. Chung, MD2, Joseph Wenke, PhD2, Charles Guymon, MA2, Garth Ehrlich, PhD3, Rachel Kreft, RN, BS3, J. William Costerton, PhD3, Joseph Hsu, MD2, Katrin Mende, PhD4, Duane Hospenthal, MD, PhD5 and Clinton K. Murray, MD1, (1)San Antonio Military Medical Center, Fort Sam Houston, TX, (2)US Army Institute of Surgical Research, Fort Sam Houston, TX, (3)Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA, (4)Infectious Disease Clinical Research Program, Fort Sam Houston, TX, (5)San Antonio Military Medical Center, MCHE MDI 7 East, TX

Disclosures:

H. Yun, None

H. Crouch, None

B. Thompson, None

M. Castillo, None

K. K. Chung, None

J. Wenke, None

C. Guymon, None

G. Ehrlich, None

R. Kreft, None

J. W. Costerton, None

J. Hsu, None

K. Mende, None

D. Hospenthal, None

C. K. Murray, None

Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.