1145. Using Predictive MHC Class I Binding Algorithms to Identify Novel Human CD8+ T-cell Epitope Candidates in Respiratory Syncytial Virus
Session: Poster Abstract Session: Innate and Adaptive Immunity to Infections
Saturday, October 22, 2011
Room: Poster Hall B1
Handouts
  • IDSA 2011 Poster.pdf (864.8 kB)
  • Background: Respiratory syncytial virus (RSV) is the most important pathogen for lower respiratory tract infections (LRI) in infants and children worldwide, and there is no licensed vaccine. In infants, CD8+ T cell responses mediate viral clearance. For this reason CD8+ T-cell epitopes are a promising alternative target for RSV vaccine development. RSV antigenic subgroups A and B cocirculate and cause disease. Novel B strains are emerging, yet the T cell responses to RSV B remains largely unexplored. While some CD8+ T-cell epitopes of RSV have been identified in mice and humans, no studies have used diverse genotypic strains of RSV to screen for epitopes. We hypothesized that strain sequence variability may play a key role in immune response. Methods: Using multiple MHCI binding algorithms with different prediction methods we compare epitope predictions from two diverse genotypic strains of RSV, A2 and B1 (B WV/14617/85), for HLA-A2 individuals. Results: We demonstrate that RSV proteins of greater sequence variability between different genotypic strains of virus predict locations of strain specific epitopes. The most divergent sequence between A2 and B1 is found within the G protein (53% sequence homology) which had  a predicted epitope diversity of 81% while the most conserved protein, N (96% sequence homology),  had a predicted epitope diversity of 23%.   Conclusion: This method can be used to predict conserved and subgroup specific protective human CD8+ T-cell RSV epitopes to be tested in further immunologic studies.  

     


    Subject Category: E. Innate and adaptive immunity to infections, including vaccine immunology

    Craig Shapiro, MD, Sujin Lee, PhD and Martin Moore, PhD, Pediatrics, Emory University School of Medicine, Atlanta, GA

    Disclosures:

    C. Shapiro, None

    S. Lee, None

    M. Moore, None

    Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.