277. Antibacterial Effect of Oxidized Cellulose and Povidone Iodine: in Vitro Study and Potential Applications in the Prevention of Surgical Site Infection
Session: Poster Abstract Session: Antimicrobial Therapy: Clinical Studies
Friday, October 21, 2011
Room: Poster Hall B1
Handouts
  • POSTER IDSA TOUATI 1.pdf (1018.4 kB)
  • Background: Reduce surgical site infections (SSI) remains a daily concern of all surgical teams and hospital hygiene. However, despite the meticulous care taken by all actors, there is a border area between the retractor and the margins of surgical incision, which is fundamental and must be the subject of attention. This area, eminently critical, is  usually "protected" by a surgical drape, which is in most centers, a simple sterile woven drape, disposable with no antibacterial activity. The aim of our study is to analyze and compare in vitro antibacterial efficacy of oxidized cellulose, regenerated or not (ROC, NROC), alone or in combination with povidone-iodine (PVI) which is commonly used in the site procedure.

    Methods: Six groups were made: Gr.1: sterile gauze, Gr.2 : ROC, Gr.3:NROC, Gr.4: sterile gauze impregnated with 93ul of PVI, Gr 5: ROC + 93ul PVI, Gr 6 : NROC + 93ul PVI. Each patch (4cm2)of tissue tested was placed in a Petri box using the methodology of the standard NF EN ISO 20645 with an inoculum of 1 ml containing 5x108 CFU / ml for each strain. The 7 strains tested were: Ps aeruginosa, St. aureus, St. aureus Methi, E. Coli, Enter. hirae, Enterococcus faecium Vancore Gen A and B. Enterococcus faecalis Vanco-resistant Gen.B. Evaluation was based on the absence or presence of bacterial growth in the area of contact between tissue and agar, and the extent of the inhibition zone around the tissue tested.

    Results: All controls (Gr 1 and 4) with or without PVI has no antibacterial effect. The effect of ROC is satisfactory on all strains except for Ps aeruginosa with or without PVI, but no culture in the sample is observed. For each strain tested, the effect of PVI on the ROC increases the inhibition zone in a ratio of 3 to 15 depending on the strain, indicating a real bactericid synergy. For each strain tested, the effect of PVI on the NROC increases the inhibition zone in a ratio of 2 to 18. The acidic pH induced by oxidized cellulose can enhance the efficiency of the PVI, is the most likely hypothesis.

    Conclusion: The combination of PVI and oxidized cellulose gives indisputable synergistic results on all different strains. Their routine use in surgery, but separately for different indications, may propose to be given in combination on the banks of the incision to fight against SSI.


    Subject Category: A. Antimicrobial agents and Resistance

    Gilles Touati, M.D., Ph.D., Cardiac Surgery, South University Hospital, Amiens, France, Misbaou Barry, M.D., Ph.D., cardiac Surgery, south University Hospital, amiens, France, M Belmekki, m.D., South University Hospital, Amiens, France, M. Biendo, M.D., Microbiology, South University Hospital, amiens, France and Philippe Strohl, Ph.D., Institut de Recherche Microbiologique, Mitry Mory, France

    Disclosures:

    G. Touati, None

    M. Barry, None

    M. Belmekki, None

    M. Biendo, None

    P. Strohl, None

    Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.