1308. Detection and Phylogenetic Analysis of Coronavirus from Ducks and Chickens in Southern China
Session: Poster Abstract Session: Viral Immunology and Pathogenesis
Saturday, October 22, 2011
Room: Poster Hall B1
Background: The identification of SARS coronavirus as the cause of severe acute respiratory syndrome (SARS) and the detection of closely related coronaviruses in bats and palm civets suggest that animals may be the natural reservoirs of coronaviruses, and occasional interspecies transmission from animals to humans can result in severe zoonotic disease outbreaks. Coronavirus surveillance in different animal hosts especially those in close contact with human beings is vital for the preparedness of the next disease outbreak.

Methods: In this study, rectal swabs were obtained from apparently healthy ducks (n=206) and chickens (n=216) in markets from southern China, and RT-PCR using a set of universal primers targeting the most conserved region in the genome of coronavirus was conducted.

Results: A total of 14 (6.7%) samples were detected positive in ducks while none of the chicken specimens showed positive results. Sequencing of these 439-bp of PCR products revealed that all amplicons were derived from the RNA-dependent RNA polymerase gene of coronavirus. Phylogenetic analysis suggested that these viruses belonged to three different subgroups of coronavirus in group III, and these novel variants may represent new members in this family.

Conclusion: Our results demonstrate that highly divergent coronaviruses are prevalent in domestic birds in southern China, and they could possibly pose continuous threat to poultry as well as human beings.


Subject Category: V. Virology including clinical and basic studies of viral infections, including hepatitis

Yuansheng Liu, MD, PhD1, Manxiong Cao2, Huachen Zhu3 and Yi Guan3, (1)The Chaonanminsheng of Shantou Hospital, Shantou, Guangdong, China515144, Shantou, China, (2)The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China, Shantou, China, (3)Shantou University Medical College, Shantou, China

Disclosures:

Y. Liu, None

M. Cao, None

H. Zhu, None

Y. Guan, None

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