602. Efficacies  of Vancomycin, Tigecycline alone and the combination of Tigecycline plus Daptomycin on Multi-Drug Resistant Organism Induced War Wound Rabbit Osteomyelitis Model
Session: Poster Abstract Session: Novel Antimicrobial Agents
Friday, October 21, 2011
Room: Poster Hall B1
Handouts
  • 602_LiyanYin.pdf (211.7 kB)
  • Background: We evaluated the treatment efficacy of Vancomycin (VAN), Tigecycline (TIG) alone and the combination of Tigecycline plus Daptomycin (TIG+DAP) on Multi-Drug Resistant Organisms (MDRO) induced war wound rabbit osteomyelitis.

    Methods: Localized osteomyelitis was induced in New Zealand White rabbits by percutaneous injection of MDRO (Pseudomonas aeruginosa Klebsiella pneumoniaAcinetobacter baumannii,  MRSA ) into the left tibia intramedullary cavity. At 2-wk post-infection, rabbits with radiographically-confirmed localized proximal tibial osteomyelitis were randomized into 4 treatment groups (n = 15 per group): untreated, VAN (sc, 30 mg/kg, q12h), TIG (sc, 5 mg/kg, q12h), or TIG (sc, 5 mg/kg, q12h)+DAP (sc, 25 mg/kg, q24h). Radiographs of tibias were taken at 6 and 8 weeks post-infection. Rabbits were euthanized at 8 weeks post-infection and tibias harvested.  Bone matrix and marrow from the left tibia were cultured and bacterial counts recorded.

    Results: All untreated control tibias and those treated with VANC showed positive cultures.  All TIG treatment group yielded positive cultures.  All TIG/DAP combination treatment groups showed positive cultures.

    Conclusion: The results suggest that VAN and TIG alone and the combinations of TIG/DAP were not effective in the treatment of MDRO-induced war wound rabbit osteomyelitis.


    Subject Category: B. Bacterial pathogenesis, studies in animal models, molecular pathogenicity

    Li-Yan Yin, MD1, Jason Calhoun, MD1, M.M. Manring, PhD1, Ian Skinner2, Paul Hutchinson3, Randy Roberts1, Yi Ding3, Gan Tong3 and Clinton K. Murray, MD4, (1)Orthopaedics, The Ohio State University, Columbus, OH, (2)Western University of Health Sciecne, Pomona, CA, (3)The Ohio State University, Columbus, OH, (4)Brooke Army Medical Center, Ft. Sam Houston, TX

    Disclosures:

    L. Y. Yin, None

    J. Calhoun, None

    M. M. Manring, None

    I. Skinner, None

    P. Hutchinson, None

    R. Roberts, None

    Y. Ding, None

    G. Tong, None

    C. K. Murray, None

    Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.