591. Combination Topical Agent containing Silver Sulfadiazine,Tetrahydrocurcuminoid and Zinc salts Promotes Wound Healing in Rat Skin Wound model
Session: Poster Abstract Session: Novel Antimicrobial Agents
Friday, October 21, 2011
Room: Poster Hall B1
Background: 

Topical antimicrobial Silver Sulfadiazine (Silvadene) is standard of care in the treatment of burn wounds. Zinc as an essential micronutrient, has a well described role in wound repair by serving as a cofactor in numerous transcription factors and enzyme systems. Tetrahydrocurcuminoid (THC) has been shown to have antiinflammatory and antioxidant properties. It is therefore reasoned that topical combination of Silver Sulfadiazine,THC and Zinc can act synergistically to promote wound healing especially in chronic nonhealing wounds and diabetic ulcers.

Methods: 

Using a rat skin wound healing model, the animals were grouped into three treatment groups of six rats in each group: Placebo cream, Silver Sulfadiazine cream, and Silver Sulfadiazine, THC and Zinc salts (Silvazin) respectively. A 3x3 cm full thickness skin wound was created and different topical agents applied. Wound size was measured on day 7 and day 14 and percentage reduction in wound size was calculated. Wound biopsies were also taken on day 7 and day 14. A second set of experiment was conducted where the wound was infected with Staph. aureus and wound size and bacterial counts from wound cultures were determined .T-test (p<0.05) was used to compare the wound healing rates.

Results: 

Silvazin had the best wound healing properties on Day 7 and Day 14 in both non infected and infected groups. Silvazin also showed statistically significant wound healing at Day 14 in non infected wound compared to Silvadene alone (P= 0.012) or Placebo alone (P= 0.0006).  Semiquantitative swab cultures and quantitative wound biopsy cultures also demonstrated the least bacterial load in Silvazin treated skin wounds. Wound histopathology and immunohistochemical assessment of angiogenesis and cellular proliferation showed the best healing process in Silvazin group.  

Conclusion:

Our preliminary data demonstrates enhanced wound healing properties of the topical cream  containing Silver Sulfadiazine, THC and Zinc salts when compared to Silver Sulfadizine alone. This novel composition could prove an effective treatment modality for debilitating chronic diabetic wounds decubitus ulcers and burn wounds. We are studying diabetic and ischemic animal wound models presently to validate these findings.


Subject Category: A. Antimicrobial agents and Resistance

Paritosh Suman, MBBS, Hari krishnan Ramchandran, PhD, Sossy Sahakian, BS, Mark Hardy, MD and Shanta Modak, PhD, Department of Surgery, Columbia University , New York, NY

Disclosures:

P. Suman, None

H. K. Ramchandran, None

S. Sahakian, None

M. Hardy, None

S. Modak, None

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