1024. Clinical assessment of nanotechnology and miniaturized magnetic resonance to detect candidemia in hospitalized patients
Session: Poster Abstract Session: Diagnosis of Bloodstream / Cardiovascular Infections
Saturday, October 22, 2011
Room: Poster Hall B1
Background:  A delay in antifungal therapy increases mortality rates in patients with candidemia.  A new, rapid diagnostic using nanotechnology and miniaturized magnetic resonance (NMMR) technology is currently being developed to identify Candida species directly from whole blood.  However, whether this NMMR platform could improve the time to diagnose Candida species is unknown.   The purpose of this pilot study was to compare the time to identification of Candida species from patients with systemic candidiasis using NMMR compared to traditional microbiology techniques.  A secondary objective was to assess the time to blood sterility using the NMMR platform.

Methods: Green and purple top vacutainer blood tubes were collected from hospitalized patients with candidemia within six hours of the collection time of the positive blood culture positive for Candida species and for the subsequent 5 days while hospitalized.  Candida was identified from the clinical microbiology laboratory using automated culture techniques (Vitek).  Vacutainer tubes were shipped on dry ice to T2 Biosystems for NMMR testing.  The time to identification using traditional vs. NMMR diagnostics was compared. 

Results:  Vacutainer tubes from five patients (four male / 1 female) aged 71± 5 years with candidemia were collected.  Candida species identified by the clinical microbiology laboratory included C. albicans (n=2), C glabrata (n=2), and C. parapsilosis (n=1).   Candida species were correctly identified from both the green and purple top vacutainers collected on the day of blood cultures from all five patients using NMMR.  Time to identification of Candida species averaged 1.8±0.4 days (range: 1.2-2.4 days) using traditional microbiology techniques and 2.25 hours (range 2.15-2.35 hrs) using NMMR (p<0.05).   Blood tubes were positive for the next two days in patients with C. albicans and greater than five days in patients with C. glabrata and C. parapsilosis.    

Conclusion:   A rapid diagnostic platform using NMMR technology was able to significantly decrease the time to identification of Candida species in hospitalized patients with candidemia.  This diagnostic tool may improve the timeliness of antifungal therapy and may aid in clinically monitoring patients.


Subject Category: D. Diagnostic microbiology

Kevin W. Garey, PharmD, M.S.1, Dhara Shah, PharmD2, Musarat Habib, BS2, Todd M. Lasco, PhD3, Lori Neely, Ph.D.4, Mark Audeh4, Amy Castonguay4, N. A. Phung4, T. Wang4 and Tom Lowery, Ph. D.4, (1)Clinical Sciences and Administration, University of Houston College of Pharmacy, Houston, TX, (2)University of Houston College of Pharmacy, Houston, TX, (3)Clinical laboratory, Saint Lukes Episcopal Hospital, Houston, TX, (4)T2 Biosystems Inc., Lexington, MA

Disclosures:

K. W. Garey, T2 Biosystems: Grant Investigator, Research grant

D. Shah, None

M. Habib, None

T. M. Lasco, None

L. Neely, T2 Biosystems: Employee, Salary

M. Audeh, None

A. Castonguay, None

N. A. Phung, None

T. Wang, None

T. Lowery, T2 Biosystems: Employee, Salary

Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.