397. Emerging in Mexico of carbapenem resistance  KPC-carrying Acinetobacter baumannii
Session: Poster Abstract Session: Gram Negative Infection - Epidemiology and Prevention
Friday, October 21, 2011
Room: Poster Hall B1
Background: Our nosocomial pathogens surveillance system had detected an increase of carbapenem resistant isolates mainly klebsiella spp (KPC) The concern has been an increasing in carbapenem resistance  in Acinetobacter baumannii. isolates and the potential horizontal transfer of antibiotic resistance genes interspecies.   

Methods:Since 2009 we have detected 31 cases of A. baumanni infection.  For their identification and susceptibility test we used an automated system  (MicroScan Walkaway's).  From January 2011 we started the KPC detection in these isolates by CHROMagar KPC and their confirmation by PCR for the blaKPC gene.

Results:From 2009 to may 2011, we detected 31 A. baumanni isolates from different clinical samples: sputum (22), blood (6) and other biological fluid (3); 14 (45%) isolates have carbapenem resistance phenotype. These isolates were identified in the last 5 months. Of the 31 cases, 27 isolates grown in CHROMAGAR KPC (87%) and 7 of these were blaKPC gene positive The rate of KPC by PCR in our isolates was 22. 5 %, and the rate of KPC-producing carbepenem resistence among CROMOAgar positive isolates was 25.6%. 2 isolates with phenotype carbapenem susceptible had the blaKPC gene.

Conclusion:Recently, we have identified an increasing of carbapenem-resistant A. baumannii infections. Of these at least an quarter are KPC-carrying isolates.  According to our surveillance, Klebsiella KPC was first detected in our hospital, so, these findings suggest the horizontal transmission of carbapenem resistance genes between different nosocomial pathogens.


Subject Category: D. Diagnostic microbiology

Patricia Rodriguez Zulueta1, Saul Lopez Campos2, Javier Reyes Mar3, Francisco Velez Perez3, Luis Ochoa Cabrera4, Luis Fernandez Maya4, Cudberto Espinoza Lopez3, Ricardo Valdez Castro5, Amy Peralta Prado6, David Moncada Baron7, Sara Arroyo Escalante5, Rigoberto Hernandez Castro4 and RafaeL Valdez Vazquez7, (1)Infectology , Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (2)Instituto Nacional de Salud Publica , Mexico City, Mexico, (3)Infectology , Hospital Manuel Gea Gonzalez , Mexico City, Mexico, (4)Ecology and pathogens, Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (5)Infectology, Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (6)Internal medicine, Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (7)Infectology, Hospital Manuel Gea Gonzalez , Mexico City, Mexico

Disclosures:

P. Rodriguez Zulueta, None

S. Lopez Campos, None

J. Reyes Mar, None

F. Velez Perez, None

L. Ochoa Cabrera, None

L. Fernandez Maya, None

C. Espinoza Lopez, None

R. Valdez Castro, None

A. Peralta Prado, None

D. Moncada Baron, None

S. Arroyo Escalante, None

R. Hernandez Castro, None

R. Valdez Vazquez, None

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