1127. Detection of Respiratory Virus Infection using Respiratory Virus Panel Assay during Outbreaks of Pandemic Influenza A/H1N1 2009 in Thailand
Session: Poster Abstract Session: Influenza and H1N1 Diagnosis, Epidemiology, and Viral Outcome
Saturday, October 22, 2011
Room: Poster Hall B1
Handouts
  • Poster IDSA.pdf (253.8 kB)
  • Background: Little is known about prevalence and seasonal distribution of respiratory virus infections in Thailand. During the outbreaks of pandemic influenza A/H1N1 2009 virus, we introduced use of the xTAG™ Respiratory Viral Panel (RVP) assay which simultaneously detects and identifies nucleic acids of multiple respiratory viruses from clinical specimen of individuals with respiratory tract infections.

    Methods: Retrospective study of the nasopharyngeal swab specimen obtained from patients with symptomatic respiratory tract infection during September 2009 to October 2010. All of the specimens were tested for influenza B and other respiratory viruses using RVP assay.  Prevalence of the non influenza A respiratory (n-FA) virus infections were compared with those of real-time PCR confirmed influenza A virus during the same period.

    Results: One hundred and sixty-two (61.8%) patients were tested positive for at least one type of n-FA virus. Compared to influenza A virus, the prevalence of n-FA virus were approximately 4 times lower. Respiratory syncytial virus (RSV) (25.2%) and rhinovirus (25.2%) were the most common type of n-FA viruses identified, followed by influenza B (13.7%) and parainfluenza virus (13%).  Emerging viruses including human metapneumonvirus and bocavirus were noted in a much less frequency (10%). RSV and influenza B virus infections were peak during mid-end of rainy season while other viruses occurred without seasonal predilection.  RSV, influenza B virus, parainfluenza virus and adenovirus primarily affected children of less than 18 years of age while rhinovirus occurred in mixed age groups. However, the positivity rate of rhinovirus was highest in adult population.

    Conclusion: The prevalence of infection from n-FA virus was lower than influenza A virus during the outbreaks of pandemic influenza A/H1N12009. However, the number of n-FA infected cases was not neglectable, particularly among children. This preliminary data merits further study with regard to cost-effective analysis, including the impact of the test on oseltamivir use during the non-pandemic setting. 


    Subject Category: V. Virology including clinical and basic studies of viral infections, including hepatitis

    Takol Chareonsirisuthigul, PhD. MD.1, Siriorn P. Watcharananan, MD2, Chutatip Srichunrusami3 and Wasun Chantratita, Ph.D.3, (1)Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Bangkok, Thailand, (2)Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Bangkok, Thailand, (3)Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Bangkok, Thailand

    Disclosures:

    T. Chareonsirisuthigul, None

    S. P. Watcharananan, None

    C. Srichunrusami, None

    W. Chantratita, None

    Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.