1289. Molecular Serotyping in an Outbreak of Acute Hemorrhagic Conjunctivitis
Session: Poster Abstract Session: Viral Epidemiology
Saturday, October 22, 2011
Room: Poster Hall B1

Adenoviruses are non-enveloped, double-stranded DNA viruses with icosahedral capsids. Adenoviral conjunctivitis is a major cause of ocular morbidity and mainly caused by HAdV-3(in HAdV-B), HAdV-4 (in HAdV-E), HAdV-8, -19 and -37(in HAdV-D). Acute hemorrhagic conjunctivitis is associated with enteroviruses. Among these, Coxsackie A-24 variant (CA-24) and Enterovirus-70 (EV-70) are known to cause epidemics and pandemics. The main objective of this study was to determine the types of adenoviruses and enteroviruses causing conjunctivitis during 2010 epidemics in Korea. 


A total of 121 conjunctival scrapings were taken from community-based clinics during 2010 in Jeonju, Korea. The inferior tarsal conjunctival and fornix were swabbed with a Dacron swab soaked with 2 ml of Eagle’s viral transport medium. Conjunctival swab specimens were inoculated onto cell lines (A549, 293A, RD, Hep-2 cell), and the cells were observed for the development of cytopathic effects. The serotypes were determined by PCR-Sequencing of the hexon gene in adenovirus and VP1/VP3 gene in enterovirus.


Of the 121 samples, 81 (66.9%) had adenoviruses detected by polymerase chain reaction (PCR). Thirty-seven (45.7%) were adenovirus type 8, 16(19.8%) were adenovirus type 11, 11(13.6%) type 19, 7(8.6%) were Coxsakievirus A24 , 6(7.4%) were adenovirus type 3 and 37(4.9%) type 3.


This study determined the types of adenoviruses causing acute hemorrhagic conjunctivitis in Korea. Direct sequencing techniques is an efficient, accurate, and rapid means of diagnosing adenoviral hemorrhagic conjunctivitis. The most common causes of adenoviral hemorrhagic conjunctivitis during 2010 epidemics in Korea were adenovirus type 8, 11, 19 and CA24.

Subject Category: V. Virology including clinical and basic studies of viral infections, including hepatitis

Chang Seop Lee, MD, Chonbuk National University, Jeonju, South Korea, Ju-Hyung Lee, Chonbuk National University Medical School, Jeonju, South Korea and Cheon-Hyeon Kim, Jeollabukdo Institute of Health & Environmental Research, Imsil, South Korea


C. S. Lee, None

J. H. Lee, None

C. H. Kim, None

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