1165. Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Pediatric Patients in Cartagena de Indias, Colombia
Session: Poster Abstract Session: Staphylococcus aureus Infections in Children
Saturday, October 22, 2011
Room: Poster Hall B1
Handouts
  • POSTER NREYES IDSA.pdf (931.6 kB)
  • Background:  Staphylococcus aureus (SA) is a major cause of skin and soft tissue infections such as furuncles and abscess and less frequently it causes bone and joint infections. Published reports of SA infections in the north coast of Colombia are limited. Here, we describe for the first time the molecular features of SA isolated from infections in pediatric pacient attended the main children’s hospital in Cartagena-Colombia

    Methods:  A total of 178 clinical isolates of SA were collected prospectively from October 2009 through December 2010. SA isolates were subjected to multiplex PCR to assess the presence of genes for PVL and five staphylococcal virulence factors. SCCmec type and PFGE typing were established in MRSA isolates. Susceptibilities for eleven antibiotics were determined and clinical outcomes and complications were described.

    Results: Seventy-eight (43,8%) infections were caused by MRSA isolates, of the which 76 (97,4%) have the molecular features of community associated MRSA (CA-MRSA). In CA-MRSA isolates, PVL, seq and bsa genes were detected in 79,5%, 75,6% and 75,6%, respectively; and all isolates were related clonal complex 8. Subtype IVc was present in 72,5% of MRSA isolates with SCCmec IV  and  subtype IVa in 7,7%. Genes for PVL were present in 68 isolates (68%) of methicillin-susceptible SA (MSSA) isolates. Resistance to tetracycline was present in 19,2% of SA isolates, erythromycin in 5.1%, and clindamycin in 2.5%. A total of 82% patients had skin and soft tissue infections (43,1% caused by MRSA), 4,5% bone infections (37,5% caused by MRSA) and 13,5 % sepsis (30% caused by MRSA). Admission to intensive care unit was required for 6.1% of patients and 1.7% of patients died.

    Conclusion:a significant proportion of SA pediatric infections in Cartagena are caused by CA-MRSA. However, MSSA remains an important pathogen responsible for community associated pediatric infections. The burden of community associated infections caused by this pathogen is increasing worldwide warning the healthcare systems about the emergence and dissemination of these infections.


    Subject Category: P. Pediatric and perinatal infections

    Niradiz Reyes, PhD1, Alfonso Bettin, MSc1, Juan Rebollo, BSc1, Betsy Castro, BSc2, Alejandro Marquez2 and Javier Escobar, MSc2, (1)Universidad de Cartagena, Cartagena, Colombia, (2)Universidad El Bosque, Bogota, Colombia

    Disclosures:

    N. Reyes, None

    A. Bettin, None

    J. Rebollo, None

    B. Castro, None

    A. Marquez, None

    J. Escobar, None

    Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.