1025. Validation of Diagnostic Accuracy of Multiplex Real-Time Polymerase Chain Reaction (PCR) Test Kit (MagicplexTM Sepsis Real-Time Test) for Sepsis
Session: Poster Abstract Session: Diagnosis of Bloodstream / Cardiovascular Infections
Saturday, October 22, 2011
Room: Poster Hall B1
Background:  Sepsis is a major health problem in critically ill patients, resulting in frequent diagnostic testing. However, blood culture results are usually negative. PCR (Polymerase chain reaction) may be a powerful tool to rapidly identify bloodstream infections, compensating the loss of blood culture sensitivity.

Methods:  The MagicplexTM Sepsis Real-time Test kit was compared to yield of blood culture samples obtained from adult patients with suspected sepsis in a tertiary hospital from July 6, 2010 to May 3, 2011; clinical and laboratory information was used to determine the patient infectious status. DNA was extracted from 1 mL of EDTA treated whole-blood using the SEEPREP12TM (Cat. No. SPN1200, Seegene, seoul, Korea) and Blood pathogen KitTM (Cat. No. D-360-048, Seegene, seoul, Korea). Multiple target detection was achieved by MagicplexTM Sepsis Real-time Detection. Amplified DNA of each sample was screened and then identified, and four different fluorescent nucleotide probes were followed.

Results:  During the study period, a total of 107 specimens were collected from 101 patients. Excluding results attributable to contaminants, positive PCR and blood culture results have been found in 15 of 97 (15.5%) and 14 of 97 (14.4%) specimens, respectively, and the rate of positive results was corresponded to an overall of 22.7% (22/97) when combining both tests. The concordance rate between the two tests was 84.5%.

Conclusion:  Magicplex sepsis test served as a valuable adjunct to blood culture in adult sepsis patients.


Subject Category: D. Diagnostic microbiology

Min-Hyok Chon, MD1, Yun Seok Ryu, PhD2, Yun JI KIM, PhD2, Eun Ju Choo, MD3, Eun Jung Lee, MD4, Hong Su Kim, MD5, Sae Hwan Lee, MD5 and Jae-Bum Jun6, (1)Division of Infectious Diseases, Chonan Hospital, Soonchunhyang University, Chonan, Korea, Republic of, (2)Seegene, Seoul, South Korea, (3)Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea, (4)Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, South Korea, (5)Department of Internal Medicine, Soonchunhyang University Cheonan Hospital, Cheonan, South Korea, (6)Department of Internal Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, South Korea

Disclosures:

M. H. Chon, None

Y. S. Ryu, Seegene: Employee and Member, Salary

Y. J. KIM, Seegene: Employee and Member, Salary

E. J. Choo, None

E. J. Lee, None

H. S. Kim, None

S. H. Lee, None

J. B. Jun, None

Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.