1007. Evaluation of CHROMagar KPC for detection Klebsiella pneumoniae carbapenemase (KPC) in a general hospital in Mexico
Session: Poster Abstract Session: Detecting, Identifying, and Typing Bacteria
Saturday, October 22, 2011
Room: Poster Hall B1
Background: Carbapenem resistance in KPC-producing Klebsiella pneumoniae (KPC) is not always detected through automated systems. This is a priority in areas with high prevalence of antibiotics resistant gram negative nosocomial pathogens. Since 2010, we had detected an increasing of pandrug-resistant  Klebsiella pneumoniae. We evaluated the detection of KPC-carrying isolates by CHROMagar KPC in klebsiella spp. isolates in two Mexican General Hospitals.

Methods: We evaluated all Klebsiella pneumoniae isolates from January to December 2010. The identification and the susceptibility test were performance with an automated system (MicroScan Walkaway's 96). All isolates were inoculated in CHROMagar KPC. The genotypic confirmation was done by PCR for the blaKPC gene.

Results: 56  K. pneumoniae  isolates were evaluated. 27 (48%) of these grown in CHROMagar KPC, 26 of which were positive to blaKPC gene and they also had a carbapenem-resistant phenotype by MicroScan. The CHROMagar KPC sensitivity and specificity were 100% and 96%, respecility. Only one CHROMagar KPC positive isolate was negative for blaKPC gene.

Conclusion: The combination of a carbapenem-resistant phenotype with CHROMagar KPC medium identified all KPC-harboring Klebsiella pneumoniae in our analysis.With this results  We can recommend this method for the early detection of KPC Klebsiella pneumoniae in centers with high antibiotic resistance and low incomes.


Subject Category: D. Diagnostic microbiology

Francisco Velez Perez1, Luis Ochoa Cabrera2, Luis Fernandez Maya2, J Luis Mosqueda, MD3, Patricia Rodriguez Zulueta4, Javier Reyes Mar1, Juan Pablo Ramirez Hinojosa, MD5, Isabel Balderas Xicotencatl, MD5, David Moncada Baron6, Sara Arroyo Escalante7, Aracely Contreras Molina8, Rafael Figueroa Moreno, MD5, RafaeL Valdez Vazquez6 and Rigoberto Hernandez Castro2, (1)Infectology , Hospital Manuel Gea Gonzalez , Mexico City, Mexico, (2)Ecology and pathogens, Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (3)Universidad de Guanajuato, Leon, Mexico, (4)Infectology , Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (5)Hospital Manuel Gea Gonzalez, Mexico City, Mexico, (6)Infectology, Hospital Manuel Gea Gonzalez , Mexico City, Mexico, (7)Infectology, Hospital Manuel Gea Gonzalez , Mexico City , Mexico, (8)Hospiyal Manuel Gea Gonzalez, Mexico City, Mexico

Disclosures:

F. Velez Perez, None

L. Ochoa Cabrera, None

L. Fernandez Maya, None

J. L. Mosqueda, None

P. Rodriguez Zulueta, None

J. Reyes Mar, None

J. P. Ramirez Hinojosa, None

I. Balderas Xicotencatl, None

D. Moncada Baron, None

S. Arroyo Escalante, None

A. Contreras Molina, None

R. Figueroa Moreno, None

R. Valdez Vazquez, None

R. Hernandez Castro, None

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