608. Antimicrobial efficacy of non-thermal plasma-treated alginate wound-dressing against multidrug resistant pathogens
Session: Poster Abstract Session: Novel Antimicrobial Agents
Friday, October 21, 2011
Room: Poster Hall B1
Handouts
  • 2011-10-20-IDSA Poster.pdf (5.6 MB)
  • Background: Non-thermal Dielectric-barrier discharge plasma (plasma) is currently under investigation in the field of disinfection, and wound healing. Alginate gel is already in clinical use, and no toxicity reported; but has no significant antimicrobial property either. The present in vitro studies report that non-thermal plasma-treated alginate dressing regenerate strong antimicrobial effects

    Methods: Freshly prepared calcium alginate gels were directly treated with plasma (0.6 W/cm2; 1250 Hz) over time at room air, and pathogen was inoculated on alginate dressing. After a contact time of 15 min, the pathogen cells were harvested for viability. The standard colony assay, and XTT assay were employed to evaluate biocidal efficacies. Scanning electron microscopy (SEM) was used to find gel surface-associated changes. Staphylococcus aureus, S. epidermidis, Acinetobacter baumannii, Escherichia coli, Candida albicans, and C. glabrata were tested as representative pathogens (106 - 109 CFU/ml). The thickness of dressing was 1 mm and distance between plasma probe and the gel surface was kept 1 mm constant

    Results: Plasma treatment of alginate gel generated a strong biocidal property, and was able to completely inactivate (sterilize) all the pathogens tested (including fungi) in 15 second of treatment. Optimization experiments revealed that 15 min contact time is sufficient for complete inactivation of pathogens even at 108 CFU/ml. In 1 min, 109 CFU/ml were inactivated completely. The biocidal effect is retained in dressing for more a month at room temperature. SEM imaging revealed no damage of gel surfaces even upon the plasma treatments of 3 min. Further studies on extended self-life (beyond one month) and wound tissue and animal toxicity are underway

    Conclusion: Plasma treatment of alginate dressing generates powerful antimicrobial properties, and this effect is retained more than a month; Treated dressing inactivated resistant wound pathogens in very high concentrations (109 CFU/ml) in less than a minute. Together with alginate dressing’s superior wound healing and protecting properties, this technology will help in control of wound infection to enhance further healing


    Subject Category: N. Hospital-acquired and surgical infections, infection control, and health outcomes including general public health and health services research

    Alexander Poor, MD1, Utku Ercan, BS2, Gregory Fridman, PhD2, Alexander Fridman, PhD2, Gary Friedman, PhD2, Ari Brooks, MD1 and Suresh Joshi, MD, PhD1, (1)Drexel University College of Medicine, Philadelphia, PA, (2)Drexel University College of Engineering , Philadelphia, PA

    Disclosures:

    A. Poor, None

    U. Ercan, None

    G. Fridman, None

    A. Fridman, None

    G. Friedman, None

    A. Brooks, None

    S. Joshi, None

    Findings in the abstracts are embargoed until 12:01 a.m. EST Thursday, Oct. 20 with the exception of research findings presented at IDSA press conferences.