929. Effectiveness of Ultraviolet (UV)-C light against C. difficile, Acinetobacter, and Vancomycin-Resistant Enterococci in the Hospital Environment
Session: Poster Abstract Session: Disinfection and Sterilization
Friday, October 19, 2012
Room: SDCC Poster Hall F-H
  • Phase I poster FINAL.pdf (884.6 kB)
  • Background: UV-C light is efficacious at reducing bacteria in controlled, experimental conditions.  In addition, previous work from our group has established that UV-C decreased MRSA from surfaces of hospital rooms, but effectiveness against C. difficile, Acinetobacter, and VRE has not been established in the hospital environment.

    Methods: Patients with infection due to C. difficile, Acinetobacter, or VRE were identified at two tertiary-care centers using standard infection control surveillance.  After discharge but prior to terminal room cleaning by environmental services, 3 environmental cultures were obtained from each of 5 pre-specified locations throughout the room using Rodac plates, including “high touch areas” and bathroom (n=15 Rodacs/room).  UV-C light was then administered to the room until a bactericidal dose was achieved (≥ 12,000µWs/cm2 for vegetative bacteria and ≥ 22,000µWs/cm2 for spores).  Following UV-C administration, 3 environmental cultures were obtained from the same 5 locations (n=15). Growth of C. difficile, Acinetobacter, and VRE were quantified and colony counts were compared “before” and “after” UV-C light administration.

    Results: In total, we analyzed 26 rooms that housed patients with VRE, 17 rooms with C. difficile, and 2 rooms with Acinetobacter.  A total of 719 CFUs of VRE were identified before UV-C and 15 were identified after (97.9% reduction).  A mean of 1.39 colony forming units (CFUs)/Rodac of VRE was identified before UV-C compared with a mean of 0.023 CFUs/Rodac after UV-C (92.3% reduction).  Similarly, 52 CFUs of Acinetobacter were identified before UV-C and only 1 CFU was identified after (98.1% reduction).  A mean of 1.73 CFUs/Rodac of Acinetobacter  was identified before UV-C while a mean of 0.033 CFUs/Rodac was identified after UV-C (87.1% reduction).  Importantly, no C. difficile was isolated from environmental cultures before or after UV-C light administration.

    Conclusion: UV-C light is effective in killing VRE and Acinetobacter in the hospital environment.  Work to improve the sensitivity of environmental culturing of C. difficile is ongoing.

    Deverick J. Anderson, MD, MPH1, Maria Gergen2, David J. Weber, MD, MPH, FIDSA, FSHEA2, Emily Smathers, MPH3, Daniel J. Sexton, MD, FIDSA3, Luke F. Chen, MBBS, MPH, CIC, FRACP3, William Rutala, PhD, FSHEA2 and the CDC Prevention Epicenters Program, (1)Duke Infection Control Outreach Network, Duke University Medical Center, Durham, NC, (2)Department of Hospital Epidemiology, University of North Carolina Health Care, Chapel Hill, NC, (3)Duke Infection Control Outreach Network, Duke Univ. Med. Ctr., Durham, NC


    D. J. Anderson, Merck, Inc.: Grant Investigator and Speaker's Bureau, Grant recipient and Speaker honorarium
    UpToDate Online: Author, Licensing agreement or royalty
    Robert Wood Johnson Foundation: Grant Investigator, Grant recipient

    M. Gergen, None

    D. J. Weber, Clorox: Consultant, Consulting fee
    CDC: Grant Investigator, Research grant

    E. Smathers, None

    D. J. Sexton, UpToDate: Consultant and Independent Contractor, Licensing agreement or royalty

    L. F. Chen, Merck: Investigator, Research grant
    Cubist Pharmaceuticals: Speaker's Bureau, Speaker honorarium
    Optimer Pharmaceuticals: Speaker's Bureau, Speaker honorarium
    Medscape: Independent Contractor, Consulting fee
    UpToDate: Independent Contractor,

    W. Rutala, Clorox: Consultant, Consulting fee
    Advanced Sterilization Products: Consultant, Consulting fee
    3M: Speaker, Speaker honorarium

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