1720. N-acetylcysteine attenuates the inflammatory response in the cervix following intrauterine lipopolysaccharide (LPS) injection in cluster of differentiation-1 (CD-1) mice
Session: Poster Abstract Session: Studies of the Interface of Host-Microbial Interaction
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C
Posters
  • N-acetylcysteine (NAC).pdf (576.7 kB)
  • Background: Maternal immune activation (MIA) is associated with preterm birth (PTB) and abnormal neurologic outcome. We have previously demonstrated a reduction in preterm birth in this model in animals treated with N-acetylcysteine (NAC).  We hypothesized that treatment with NAC would attenuate the cervical expression of inflammatory cytokines in an animal model for preterm birth and brain injury.

    Methods: Pregnant CD-1 mice received intrauterine LPS or saline between the first two gestational sacs within the right horn of the uterus on day 15/20. They were assigned to treatment with either NAC or saline.  Cervical tissue sampling was obtained 6 hours after LPS administration.  Quantitative polymerase chain reaction (PCR) was performed on cervical tissue to determine the mRNA expression of Il-6, Il-1b, COX-2, MMP-8, MMP-9 and iNOS.  Nonparametric testing was performed with the Kruskal-Wallis one-way analysis of variance with significance set at p < 0.05.  Pairwise comparisons were made with the Mann-Whitney U test as appropriate with significance at p < 0.05.

    Results: At an LPS dose of 100 mg/mouse, we found that the mRNA cervical expression of IL-6, COX-2 and iNOS were significantly increased in the LPS treated group compared to controls while treatment with NAC significantly decreased their expression.  MMP-8, MMP-9 and IL-1b were also elevated by LPS but NAC did not attenuate these changes.

    Conclusion: Intrauterine LPS administration leads to an increase in expression of inflammatory factors associated with cervical ripening in this murine model.  Treatment with NAC attenuates some of these effects.  NAC may be an interesting candidate for study for the prevention of cervical ripening to prevent preterm delivery.

    Jingmei Zhang, PhD1, Bassam Rimawi, MD2 and Eugene Chang, MD1, (1)Maternal Fetal Medicine, Medical University of South Carolina, Charleston, SC, (2)Maternal Fetal Medicine and Reproductive Infectious Diseases, Medical University of South Carolina, Charleston, SC

    Disclosures:

    J. Zhang, None

    B. Rimawi, None

    E. Chang, None

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