741. Protective Effects of Pyridoxine Against Side-Effects Induced by Linezolid
Session: Poster Abstract Session: Antimicrobials: Resistance Mechanisms
Friday, October 4, 2013
Room: The Moscone Center: Poster Hall C
Background: Serious gram-positive bacterial infections are common in childhood. Linezolid is the first member of oxazolidinone group in the clinical use. It shows its effect by inhibiting the 50S ribosome. However, myelosuppression has been reported as a side-effect. In this study, we aimed to show the effectiveness of pyridoxine against linezolid induced side-effects (oxidative stress and hematological complications) on an experimental animal model. 

Methods: Forty male pediatric Spraque Dawley rats were randomly seperated into four groups. We administered 1mL of saline solution to the control group (C), 125 mg/kg/day of linezolid to second group (L), 100 mg/kg/day of pyridoxine to the third group (P) and 125mg/kg/day of linezolid plus 100mg/kg/day pyridoxine to the last group (LP) for 14 days per 12 hours. Before and after this procedure, we measured complete blood count, BUN, creatinine, ALT, AST, total and direct bilirubin values. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were measured in the erythrocytes to show oxidative stress. All analyses were made with SPSS program and p<0.05 was considered statistically significant.

Results: There was a significant decrease in white cell number in L, P and LP groups when compared to control group (p<0.0001). Serum ALT levels of L group was significantly higher than control group (p<0.0001). Activity of antioxidant enzymes SOD, GSH-Px and CAT, and level of serum MDA were higher  than control group in L group. Level of MDA, which is an indicator of lipid peroxidation,  and antioxidant enzyme activities were  decreased in L+P given group.

Conclusion: Total leukocyte count was decreased but levels of MDA, ALT and activities of SOD, GSH-Px and CAT were increased significantly in the linezolid group when compared to the control and LP groups. These findings suggest that pyridoxine has no protective effects for linezolid-induced leukopenia and increased ALT levels. On the other hand, pyridoxine may protect against the oxidative stress that occurs in erythrocytes by reducing the activity of antioxidant enzymes and MDA levels. This study, first in the literature, examined the side effects of linezolid in pediatric rats and will hopefully be a source for the future researches.

Metehan Ozen, Professor1, Yasemin Kendir, MD2, Efkan Uz3, Ramazan Yilmaz4 and Ali Ayata2, (1)Pediatrics, Suleyman Demirel University, ISparta, Turkey, (2)Sdu Deparment of Pediatrics, ISparta, Turkey, (3)Sdu Deparment of Biochemistry, ISparta, Turkey, (4)Sdu Deparment of Medical Biology, ISparta, Turkey

Disclosures:

M. Ozen, None

Y. Kendir, None

E. Uz, None

R. Yilmaz, None

A. Ayata, None

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