1718. Cellular Respiration is Inhibited in Murine Lungs Co-infected with Influenza A Virus and Respiratory Syncytial Virus
Session: Poster Abstract Session: Studies of the Interface of Host-Microbial Interaction
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C
Posters
  • SFO_Poster_Alsuwaidi.pdf (7.1 MB)
  • Background: Lung tissue mitochondrial O2 consumption (cellular respiration) is altered in infections with influenza A virus (IAV) or respiratory syncytial virus (RSV).  The fate of lung bioenergetics in co-infection with both viruses, however, is unknown.  This study was designed to investigate cellular respiration in murine lungs co-infected with IAV and RSV. 

    Methods: Taylor outbred mice were intranasally inoculated with influenza A/PR/8/34 (H1N1), with RSV A2, or with both viruses (administered sequentially 24 h apart).  On day 3 post-infection, lung fragments were analyzed for cellular mitochondrial O2 consumption.  Phosphorescence O2 analyzer that measured dissolved oxygen was used for this purpose.

    Results: O2 consumption was inhibited by cyanide, confirming the oxidation occurred in the mitochondrial respiratory chain.  The rate of respiration (mean ± SD, in µM O2 mg-1 min-1) in uninfected lungs was 0.16 ± 0.03 (n = 6), in RSV-infected lungs was 0.12 ± 0.04 (n = 6, p = 0.093), in IAV-infected lungs was 0.11 ± 0.03 (n = 6, p = 0.026), in RSV/IAV co-infected lungs was 0.11 ± 0.03 (n = 5, p = 0.052), and in IAV/RSV co-infected lungs was 0.04 ± 0.01 (n = 4, p = 0.016).

    Conclusion: In Taylor outbred mice, O2 consumption was significantly decreased in IAV-infected lungs.  The decline in respiration was more pronounced when IAV was inoculated one day prior to RSV.

    Ahmed Alsuwaidi, MD1, Abdul Kader Souid, MD, PhD1 and Steven Varga, PhD2, (1)Pediatrics, United Arab Emirates University, Al Ain, United Arab Emirates, (2)Microbiology, University of Iowa, Iowa City, IA

    Disclosures:

    A. Alsuwaidi, None

    A. K. Souid, None

    S. Varga, None

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