1781. Viral Gastroenteritis in Children in Colorado: 2006-2009
Session: Poster Abstract Session: Viral Infections; Pathogenesis and Epidemiology
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C
Posters
  • Poster ID Week-Osborne.pdf (2.0 MB)
  • Background: In the U.S., acute gastroenteritis accounts for a significant burden of medically attended illness in children under the age of five.

    Methods: Four multiplex RT-PCR assays were used to determine the incidence of seven viral agents (adenovirus, astrovirus, coronavirus, norovirus GI and GII, rotavirus, and sapovirus) in stool samples submitted for viral electron microscopy (EM) to the Children’s Hospital Colorado during a 34-month period (2006-2009).

    Results:

    Of 1587 stool samples submitted, 1082 (68%) were available for this study.  940 samples were from pediatric patients (median age = 2.97 years, 55% male). 132 were from adult patients submitted from an outside hospital during a 3-month period.  Of pediatric samples, viral nucleic acids were detected in 245 (28%) with 28 (3.1%) samples containing more than one virus.   Adenovirus was detected in 95 (10%) samples, astrovirus in 33 (4%), norovirus GI in 8 (0.9%), norovirus GII in 90 (10%), rotavirus in 49 (5.2%), and sapovirus in 2 (0.2%).  No specimen was detected by the coronavirus assay.  Several immunocompromised patients shed viruses for prolonged periods: norovirus for 32 weeks, astrovirus for 12 weeks, and adenovirus for 5 weeks. Of adult samples, 49 (37%) were positive for norovirus GII. PCR was more sensitive than EM, detecting 89 of 102 (87%) total adenovirus-positive samples versus 25 (25%) by EM; and 155 of 164 (95%) total astrovirus/norovirus-positive samples versus 23 (14%) by EM. EM (55 of 84, 66%) detected slightly more rotaviruses than did PCR (50, 60%).

    Conclusion: Noroviruses and adenoviruses are common causes of gastroenteritis in children.  Immunocompromised patients can be infected with multiple viruses and shed viruses for prolonged periods.  Our data support the superiority of RT-PCR compared to EM for the diagnosis of viral gastroenteritis.  Continued efforts at prevention and therapeutic interventions for noroviruses are warranted.

    Christina Osborne, BA1, Aaron Montano, BS1, Christine C. Robinson, PhD2, Stacey Schultz-Cherry3 and Samuel R. Dominguez, MD, PhD4, (1)Pediatrics, University of Colorado School of Medicine, Aurora, CO, (2)Children's Hospital Colorado, Denver, CO, (3)Infectious Disease, St. Jude Children's Research Hospital, Memphis, TN, (4)Pediatric Infectious Diseases, University of Colorado School of Medicine and Children's Hospital Colorado, Aurora, CO

    Disclosures:

    C. Osborne, None

    A. Montano, None

    C. C. Robinson, None

    S. Schultz-Cherry, None

    S. R. Dominguez, None

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