1293. Prevalence of Escherichia coli O157:H7 Causing Children Diarrhea in Sirte, Libya
Session: Poster Abstract Session: Below the Diaphragm
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C

E. coli O157: H7 is a cause of acute infectious diarrhoea  in humans and the leading cause of haemolytic uremic syndrome, especiallyamong children in the united states. E. coli O157:H7 was known as Verotoxin producing E. Coli (VTEC).It is also called as Shiga toxin producing E. coli(STEC). More than 50 serotypes of EHEC have been isolated . E. COLIO157:H7 causes a severe disease in humans that starts with profuse diarrhea, and if not diagnosed or treated properly can lead to serious complications such as HC, HUS and thrombocytopenic purpura (TTP)We undertook a study to Estimate the incidence of Escherichia coli O157:H7 infection in diarrheic children attending Ibn Sinna Hospital, Sirte


124 stool specimens of diarrheic children were collected at paediatric department in Ibn Sinna teaching hospital in the periodfrom September 2009 to may 2010. All the samples were cultured on SMAC.Non sorbitol - fermenting colonies are tested  for the somatic O157 antigen before being confirmed as E. Coli O157.The colonies are gram stained and confirmed with O157 antiserum or latex reagent. These colonies were later biochemically confirmed as E. coli using API20E and reported as presumptively positive for E. coli O157:H7.


124 stool samples which were obtained from 124 diarrheic children contained 127 bacterial isolates belonging to 6 different genera or strains, as detected on SMAC plates Only 4 isolates were non-sorbitol fermenting colonies on SMAC agar. These sorbitol-negatives isolates were later identified serologically and biochemically as: E. COLI O157:H7, one isolate; Shigella sonnei, one isolate; Enterobcater sp, two isolates; 123 isolates fermented sorbitol on SMAC agar, and later identified serologically and biochemically as:Pseudomonas sp.


Among 124 stool specimens, only 1(0.7%) was positive for E.coli O157:H7 Magnitude of the problem due to E. coli O157:H7 appears to be not much at Sirte but, because of its ability to cause complication, E. coli O157:H7 should be screened in routine culture and identified. Out of 124 diarrhea samples tested, only four cases (3.2%) were sorbitol-negative. Although inability to ferment sorbitol has been considered the primary phenotypic criterion for identification of E. COLI O157:H7, biochemical assays were crucial for identifying E. COLI O157:H7.

Shaban R. Abdulsalam Bagar, PhD, Infectious Disease, Faculty of Medicine, Sirte - Libya, Libya


S. R. Abdulsalam Bagar, Faculty of Medicine: Consultant, Salary

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