1584. Endemic Cross-transmission of Carbapenem resistant Acinetobacter baumannii in King Fahd Hospital Jeddah: a cohort study
Session: Poster Abstract Session: Multidrug-Resistant Gram Negative Rods
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C
Background: Infections caused by Carbapenem resistant Acinetobacter baumannii (CRAB) are a major concern in king Fahd hospital Jeddah, Saudi Arabia. The magnitude of cross-transmission of the latter organism was never determined at endemic level.

Hypothesis:  a predominant genotype might be distributing within our hospital and is responsible for most of the Healthcare Associated Infections reported cases.

Aim: to determine the incidence of CRAB cross-transmission and the predominant genotype.

Methods: the study was conducted from April to December 2010. All patients who were admitted where followed until they acquired CRAB or discharged. Cross-transmission was identified by combining the genetic typing results using Random Amplified Polymorphism DNA (RAPD) and the patients' epidemiological data. Cross-transmission episodes were defined when two or more patients had indistinguishable fingerprint and had been treated in the hospital not more than 3 months apart. The direction of cross-transmission was confirmed if the incriminated pathogen was isolated from the donor before admission of the recipient; otherwise, both patients could potentially be a donor or a recipient.

Results: In total, 102 patients were followed for 5134 patients' days. Out of 104 Acinetobacter isolates collected, CRAB counted for (88.5%) 92/104 and 91 isolates were typed. Molecular fingerprinting using RAPD indentified 28 genotypes, of which 25 were sporadic and were unrelated by RPAD. 52 episodes of cross-transmission were identified with an overall incidence of 10 per 1000 patient-days.

Conclusion: On the basis of the genotyping of the isolates, we can conclude that there is a huge distribution of different genotypes of CRAB within the hospital. Cross-transmission beteen patients was determined in 3 genotypes only.  Further research is needed to detect the potential source behind the dissemination of CRAB genotypes within our hospital.

 

Muhammad Abdulrahaman Halwani, MSc, PhD, FJHMI1, Nuha AL Humidi, MSc2, Saad AL Masoudi, PhD3, Magda Aly, PhD3 and Osama Dhafar, MD4, (1)Medical Microbiology, Al Baha University, Faculty of Medicine, Al Baha, Saudi Arabia, (2)Main Lab, King Fahd Hospital, Jeddah, Saudi Arabia, (3)Microbiology, King Abdulaziz University, Jeddah, Saudi Arabia, (4)Health Affairs, Jeddah, Saudi Arabia

Disclosures:

M. A. Halwani, None

N. AL Humidi, None

S. AL Masoudi, None

M. Aly, None

O. Dhafar, None

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