1390. Effectiveness of spray application of a novel sporicidal disinfectant cleaner
Session: Poster Abstract Session: Clostridium difficile
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C

Background: STERIPLEX SD is an EPA-registered cleaner and disinfectant with sporicidal activity. In addition to use as wipe, Steriplex can be applied to surfaces using a touchless spray device. Although spraying sporicidal disinfectants provides efficient surface application, limited data are available on the effectiveness of this form of disinfectant application.

Methods: In the laboratory, we examined the efficacy of STERIPLEX solution versus a 1 to 10 dilution of household bleach for killing of Clostridium difficile spores, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA) on stainless steel carriers. To assess the effectiveness of the spray application, reductions in pathogens inoculated onto benchtop surfaces were measured after spraying with STERIPLEX directly or 6 or 12 inches from the direct line of spray and effectiveness in eliminating pathogens from hospital equipment was assessed on hospital wards.

Results: On steel carriers, STERIPLEX was as effective as bleach for disinfection of C. difficile spores, VRE, and MRSA, resulting in 6 log reductions in recovered counts of each organism. Spraying with STERIPLEX resulted in >4 log reductions in pathogens in direct line of spray (>3 minute wet contact time), whereas lower reductions were achieved as the distance from the direct line of spray increased and wet contact time decreased. Of 68 sites cultured from hospital equipment, 7 (10%) were contaminated with C. difficile or VRE, and 1 of 7 remained positive for C. difficile after spray with STERIPLEX. There was no evidence of adverse effects to surfaces after multiple applications of Steriplex and no reported complaints from nursing staff or patients.

Conclusion: STERIPLEX was as effective as bleach for in vitro killing of C. difficile spores, MRSA, and VRE. Spraying with STERIPLEX could provide an efficient and easy means of application, but effectiveness will be operator dependent because sufficient product must be applied to provide adequate contact time.

Figure: Effectiveness of STERIPLEX for disinfection of pathogens inoculated onto surfaces, stratified by distance from direct point of spray.

Abhishek Deshpande, M.D., Ph.D.1, Thriveen Mana, M.S., MBA1, Jennifer Cadnum, B.S.1, Brett Sitzlar, B.S.1, Dennis Fertelli1, Sirisha Kundrapu, M.D.1, Venkata C.K. Sunkesula, M.D., M.S2, Alex Polinkovsky, Ph.D.3 and Curtis Donskey, MD1,3, (1)Infectious Diseases, Case Western Reserve University, Cleveland, OH, (2)Case Western Reserve University, Cleveland, OH, (3)Louis Stokes Cleveland VA Medical Center, Cleveland, OH

Disclosures:

A. Deshpande, None

T. Mana, None

J. Cadnum, None

B. Sitzlar, None

D. Fertelli, None

S. Kundrapu, None

V. C. K. Sunkesula, None

A. Polinkovsky, None

C. Donskey, None

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