Methods: Patterned and un-patterned (control) thermoplastic polyurethane samples (n=4) were immersed statically in either whole blood or saline for 2 hours at 37°C, followed by a saline rinse, and inoculation with ~107 CFU/mL S. aureus (ATCC6538) in saline. The samples were incubated statically for 1 and 16 hours at 37°C before rinsing with saline and enumerating the attached cells by ultrasonication and dilution plating. Statistical analysis was conducted through t-test and ANOVA models.
Results: After preconditioning surfaces with blood, the micro-patterned surfaces reduced S. aureus colonization to a clinically relevant material by 65% (p≤0.05) after 1hr and 70% (p≤0.05) after 16hrs when compared to preconditioned un-patterned surfaces. There was no significant difference in micro-patterned surface performance across time points and preconditioning fluids (i.e. blood vs. saline).
Conclusion: The surface modification afforded by the micro-texture significantly inhibits the colonization of S. aureus after blood preconditioning on a clinically relevant material. Introduction of this micro-pattern on central venous catheters may be useful for controlling CRBSIs.
Sharklet Technologies Inc: Employee, Salary
D. Manna, Sharklet Technologies Inc: Employee, Salary
S. Reddy, Sharklet Technologies Inc: Employee, Salary