1319. Innate lymphocytes regulate eosinophil recruitment in infection and homeostasis
Session: Poster Abstract Session: Biomarkers and Correlates of Protection
Saturday, October 5, 2013
Room: The Moscone Center: Poster Hall C
Background: The classical hallmarks of helminth immunity, asthma, and allergy, coordinated by CD4+ T helper Type 2 (Th2) cells, include antibody production, mucus secretion, and innate effector cell recruitment. The cytokines interleukin (IL)-4, IL-5, and IL-13 drive these responses, but more specific understanding of the cells and interactions involved in helminth resistance or allergy pathogensis is needed to improved disease treatment.

Methods: IL-5 is the specific survival and maturation factor for eosinophils and is essential for eosinophilia. We generated IL-5 and IL-13 reporter mice to study eosinophil recruitment in response to helminth infection.

Results: Innate IL-5-producing lymphocytes were found throughout resting tissues. After infection, IL-5-producing cells increased in number and in fluorescence intensity, corresponding with local eosinophilia. Deletion of IL-5-producers and analysis of an IL-5/IL-13 co-regulation revealed different expression patterns in different effector cell types. In a CD4+ T cell transfer system, innate IL-5 was required for tissue eosinophilia.

Conclusion: Innate lymphocytes and Th2 cells produce IL-5 and IL-13. Th2 cells are required for worm clearance, however ILC2-derived IL-5 was required for tissue eosinophilia. Studies are ongoing to define the relationship between these cell types.

Jesse Nussbaum, MD1, Hong-Erg Liang, PhD1, Steven Van Dyken, PhD1, Laurence Cheng, MD, PhD2 and Richard Locksley, MD1,3, (1)Division of Infectious Diseases, University of California San Francisco, San Francisco, CA, (2)Pediatrics, University of California San Francisco, San Francisco, CA, (3)Howard Hughes Medical Institute, San Francisco, CA


J. Nussbaum, None

H. E. Liang, None

S. Van Dyken, None

L. Cheng, None

R. Locksley, None

Findings in the abstracts are embargoed until 12:01 a.m. PST, Oct. 2nd with the exception of research findings presented at the IDWeek press conferences.