Acinetobacter Baumannii: Concomitant Contamination of Air and Environmental Surfaces Based on the Anatomic Source of Colonization
We recently described the differential contamination of inpatient room surfaces among patients colonized with Acinetobacter baumannii (AB) in different bodily sources. Separately, we also found that the air is differentially contaminated based on the bodily source of AB. Thus, we now aim to concomitantly and prospectively determine the differential degree of both air and environmental contamination among patients (pts) colonized with AB in rectum versus respiratory tract.
This study was performed at a large hospital in Miami in all consecutive adult ICU pts detected to have AB on active surveillance cultures. Among AB(+) pts, air and environmental samples were obtained daily for 10 consecutive days or until discharged from hospital. Air was sampled using open blood agar plates (exchanged daily) placed above their headboards (2-ft from roof-tiles). Environmental surfaces sampled included bed rails, bedside tables, ventilator panels, and intravenous pumps. Sterile Q-tips were used to swab both, blood agar plates and environmental surfaces, immediately placed in 2mL TSB, incubated overnight, and then plated on MaConkey. AB was determined based on colony color, morphology, and final identification by Vitek II.
During 5 consecutive months, 19 AB(+) pts were identified: 13 (68.4%) colonized in the respiratory tract and 6 (31.6%) in the rectum. Of the 142 air-day samples obtained, 29-air-days (20.4%) grew AB. Air samples grew AB in 42.8% of pts colonized in the rectum and 13.1% for those colonized in the respiratory tract (p=0.0004). Regarding environmental samples, the proportions of positive samples were 17% for pts colonized in the rectum compared to 10.4% for those colonized in the respiratory tract (p=0.04).
Concomitant contamination of the ambient air and surfaces with AB seems to be higher among pts rectally colonized than among patients with respiratory colonization.
M. Sfeir, None
A. Berbel Caban, None
D. Depascale, None
T. Cleary, None
L. S. Munoz-Price, None
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