Program Schedule

Environment as a Potential Source of Fusarium spp. Invasive Infections in Immunocompromised Patients

Session: Oral Abstract Session: Fungal Infections
Friday, October 10, 2014: 3:00 PM
Room: The Pennsylvania Convention Center: 109-AB
Background: An eight-year surveillance, in our hospital, recorded the occurrence of 34 cases of invasive fusariosis in immunocompromised patients. As Fusarium spp. are widely distributed in soil, plants and in the environment, we made the hypothesis that an environmental source might be associated with the clinical cases.  Our objectives were to compare the molecular relationship of Fusarium spp. isolated from air sampling with Fusarium spp. strains obtained from blood cultures of hospitalized patients

Methods: Over one-year period, air samplings of patients’ rooms were conducted in the Bone Marrow Transplant (BMT) and Hematology wards. Air sampling was performed using selective medium for Fusarium inside a calibrated air sampler. From 2006-2013, eighteen Fusarium isolates were obtained from individual blood cultures. To identify  FSSC and non-FSSC a real-time PCR (RT-PCR) assay was designed using primes  to amplify the region 28s rRNA and probes targeted for SNP present in Fusarium spp. gene and Fusarium solani species complex.  Sequencing was performed using EF1-alpha gene amplification. Air and blood Fusarium isolates sequences were submitted to phylogenetic tree construction by Neighbor-Joining method and bootstrapped (BT) using 1,000 random replicates in MEGA5 software

Results: 118 Fusarium spp. were isolated from air in the BMT (n=35) and Hematology (n=83) wards; RT-PCR identified 13 isolates as FSSC (11.0%) and 105 isolates as non-FSSC (89.0%). The results of DNA sequencing and RT-PCR were concordant for all the isolates; additionally, sequencing identified Fusarium at species levels. Isolates from the air were mainly:  F. verticilloides (29 isolates; GFSC), F. incarnatum (23 isolates; FIESC), F. proliferatum (16 isolates; GFSC), F. chamydosporum (11 isolates; FCSC) and F. solani (13 isolates; FSSC). Isolates from blood: 13 F. cf. solani and 3 F. napiforme (GFSC). The phylogenetic tree showed a high similarity among 9 F. cf. solani isolates from the air with 8 isolates of solani from the blood (BT=99). Two F. napiforme from blood were clustered together (BT=99) with the one F. napiforme isolated from air (BT=99).

Conclusion: These data showed a high relationship between Fusarium species isolated from air and blood suggesting that the indoor hospital air may be a potential source for fusariosis in these patients.

Maria Luiza Moretti1, Ariane Busso-Lopes1, Renato Moraes1, Yasunori Muraosa2, Yuzuru Mikami2, Plinio Trabasso1, Kenichiro Tominaga3, Francheline Reichert-Lima1, Luzia Lyra1, Tohru Gonoi2, Hideaki Taguchi2, Angelica Schreiber1 and Katsuhiko Kamei2, (1)University of Campinas, Campinas, Brazil, (2)Medical Mycology Research Center, Chiba, Japan, (3)Japan International Cooperation Agency, Brasilia, Brazil


M. L. Moretti, None

A. Busso-Lopes, None

R. Moraes, None

Y. Muraosa, None

Y. Mikami, None

P. Trabasso, None

K. Tominaga, None

F. Reichert-Lima, None

L. Lyra, None

T. Gonoi, None

H. Taguchi, None

A. Schreiber, None

K. Kamei, None

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