Program Schedule

NDM-1-producing Escherichia coli Isolated from a Case Patient's Environment

Session: Poster Abstract Session: Multidrug-resistant Organisms: Epidemiology and Prevention
Thursday, October 9, 2014
Room: The Pennsylvania Convention Center: IDExpo Hall BC
  • CRE NDM poster Final.pdf (3.0 MB)
  • Background: The Oregon Health Authority identified its first New Delhi metallo-β-lactamase-1 (NDM-1)-producing Escherichia coli since mandated laboratory reporting for carbapenem-resistant Enterobacteriaceae (CRE) began during 2012. We investigated to determine risk factors, identify the source, and prevent transmission.

    Methods: A CRE case is defined as Enterobacteriaceae non-susceptible to all third-generation cephalosporins and ≥1 carbapenem tested. CRE isolates are routinely characterized via Carba NP test and PCR for carbapenemase genes. In addition, this isolate underwent pulsed-field gel electrophoresis (PFGE), strain and plasmid identification, and whole genome sequencing. We conducted laboratory and chart reviews, case and health care provider interviews, contact screenings, site visits, and environmental testing.

    Results: In November 2013, testing confirmed a NDM-1 positive, CTX-M-27-producing E. coli O25b-ST131 isolated from an outpatient shin wound culture taken from a rural Oregon county resident. Review of regional laboratory reports did not identify other NDM-positive CRE during the preceding year. The case and spouse denied international travel or visitors, and we confirmed only outpatient clinic visits during the previous year. CRE rectal swabs from the resident (N =3) and spouse (N = 1) were negative. Other possible sources such as laboratory error, food contamination, animal shedding, outpatient clinic contamination, or household water contamination were extensively reviewed.  Spice (N = 14), nutritional supplement (N = 15), indoor environmental (N = 13), outdoor environmental (N = 18), and vacuum dust (N = 4) samples were collected two months after case confirmation. An NDM-1-producing E. coli isolate indistinguishable from the resident’s isolate by PFGE was extracted and cultured from a household vacuum dust sample.

    Conclusion: Though CRE are associated with exposure to health care in CRE endemic regions, we could not identify any previously described sources in this low-prevalence region. Recovery of a matching strain from a vacuum environmental sample raises concern about the persistence of pathogenic strains in a household environment and an as yet unidentified origin.

    Genevieve L. Buser, MDCM, MSHP1, P. Maureen Cassidy, MPH1, Christopher Pfeiffer, MD, MHS2, John M. Townes, MD3, Karim E. Morey, MS, M(ASCP)4, Jaipreet Rayar, MS5, Kirthi K. Kutumbaka, PhD6, Sukkyun Han, PhD6, Cesar Nadala, PhD6, Mansour Samadpour, PhD6, Scott Weissman, MD5, Robert Vega, MS, SM(AAM)4 and Zintars G. Beldavs, MS1, (1)Acute & Communicable Disease Prevention, Oregon Health Authority, Portland, OR, (2)Infectious Diseases, Portland VA Medical Center, Portland, OR, (3)Oregon Health and Science University, Portland, OR, (4)Oregon State Public Health Laboratory, Hillsboro, OR, (5)Seattle Children's Research Institute, Seattle, WA, (6)Molecular Epidemiology Inc., Lake Forest Park, WA


    G. L. Buser, None

    P. M. Cassidy, None

    C. Pfeiffer, None

    J. M. Townes, None

    K. E. Morey, None

    J. Rayar, None

    K. K. Kutumbaka, Molecular Epidemiology Inc.: Employee, Salary

    S. Han, Molecular Epidemiology Inc.: Employee, Salary

    C. Nadala, Molecular Epidemiology Inc.: Employee, Salary

    M. Samadpour, Molecular Epidemiology Inc.: Consultant, None

    S. Weissman, None

    R. Vega, None

    Z. G. Beldavs, None

    Findings in the abstracts are embargoed until 12:01 a.m. EDT, Oct. 8th with the exception of research findings presented at the IDWeek press conferences.

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