Program Schedule

Relative Contribution of Blood and Bone Cultures in the Microbiologic Diagnosis of Staphylococcus aureus Osteomyelitis in Children

Session: Poster Abstract Session: Approach to Clinical Infections
Friday, October 10, 2014
Room: The Pennsylvania Convention Center: IDExpo Hall BC
  • Osteo poster.pdf (319.7 kB)
  • Background: Methicillin-resistant Staphylococcus aureus (MRSA) USA300 is a predominant cause of community-acquired (CA) invasive infections in the United States. Optimal treatment of S. aureus osteomyelitis requires its isolation from blood or bone cultures for determination of antimicrobial susceptibility. We determined the contribution of blood and bone cultures in the microbiologic diagnosis of osteomyelitis in the era of CA-MRSA infections. 

    Methods: Isolates and patients were identified from a prospective S. aureus surveillance study.  The database was searched from 4/2012-3/2014 for patients with the diagnosis of osteomyelitis. Medical records were reviewed and data entered on standardized forms.  Statistical analyses were performed with STATA 12.

    Results: MSSA and MRSA infection occurred in 70 (59.3%) and 48 (40.7%) children (n=118), respectively. MRSA osteomyelitis was associated with intraosseous/subperiosteal abscess (p=0.009) and/or pyomyositis/myositis (p=0.02). Blood cultures (BC) were performed in 111 of 118 (94%) patients and were positive in 61% (68/111).  In 33.8% (23/68) of cases, a BC was the only source that that yielded S. aureus.  Bacteremia did not differ between MRSA (62%) vs. MSSA (60.6%) osteomyelitis.  Surgery was performed in 75.4% (89/118) of patients.  Intra-operative cultures were positive in 93.3% (83/89) of cases and in 50.6% (42/83) it was the only positive culture. A bone biopsy was performed by interventional radiology (IR) in 12% of patients (14/118).  The IR culture was positive in 78.6% (11/14) of cases and in 36.3% (4/11) it was the only positive culture.  In 43 of 50 patients (86%) with a negative BC, bone culture (surgical or IR) led to a change in antibiotic management. 

    Conclusion: Since 2012 most cases of S. aureus osteomyelitis were caused by MSSA, continuing with a shift in epidemiology at our institution which started in 2007. The majority of patients were bacteremic, however, a bone culture (surgical or IR) played a significant role in the diagnosis and treatment of patients with negative BC.  IR bone cultures may be positive for S.aureus when BCs are negative, allowing for appropriate antibiotic treatment of osteomyelitis in the era of CA-MRSA infections.

    Jonathon Mcneil, MD, Andrea Forbes, RN, Edward O. Mason Jr., PhD, Kristina G. Hulten, PhD, Sheldon L. Kaplan, MD, FIDSA and Jesus G. Vallejo, MD, FIDSA, Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston, TX


    J. Mcneil, None

    A. Forbes, None

    E. O. Mason Jr., None

    K. G. Hulten, None

    S. L. Kaplan, Pfizer: Grant Investigator, Research grant
    Cerexa: Grant Investigator, Research grant

    J. G. Vallejo, None

    Findings in the abstracts are embargoed until 12:01 a.m. EDT, Oct. 8th with the exception of research findings presented at the IDWeek press conferences.

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