Program Schedule

991
Characteristics of Human Rhinovirus Infection in Pediatric Age

Session: Poster Abstract Session: Pediatric - Viral Studies
Friday, October 10, 2014
Room: The Pennsylvania Convention Center: IDExpo Hall BC
Posters
  • Rhino - Philadelphia Ver2.pdf (274.2 kB)
  • Background: Evaluation of the etiologic role of human rhinovirus (HRV) found in the nasopharynx of children with acute respiratory problems is difficult because it was reported that HRV can be carried by asymptomatic subjects or found in healthy children with previous disease. However, phylogenetic analysis of HRV has shown that a great number of different HRV strains can simultaneously circulate and infect people, suggesting possible continuous re-infection. Differentiation of HRV persistence from re-infection is critical and genotyping of sequentially identified strains could help at this regard. This study was planned to evaluate genetic characteristics of HRV sequentially identified in young children.

    Methods: From November 1, 2013, to March 31, 2014, a group of otherwise healthy children aged 1 to 18 months was studied. Parents were asked to collect every week a nasopharyngeal swab, to return to the Center for a monthly control visit and, when a febrile episode occurred, to complete a diary recording child’s clinical problems and medical prescriptions. RT-PCR was performed to identify HRV and positive samples were used for sequencing analysis and for reconstructing the phylogenetic tree. 

    Results: A total of 91 children was enrolled. HRV was identified in 516 swabs. All but 3 subjects had at least one swab positive in most of the cases without significant clinical problems. HRV types were identified in 392 samples (76.0%; HRV-A 151, 38.5%; -B 62, 15.8%; -C 179, 45.7%).  Positivity for ≥4 consecutive samples was found in 38 (43.2%) children. Of these, only 12 had the same strain (A12 in 3 cases and one case each for A10, A76, A89, B14, B86, B103, C1, C25, C43).

    Conclusion: This study indicates that in healthy children HRV tends to persist in the nasopharynx for a limited period of time and re-infection is the most common cause of repeated HRV identification in the same subject. Prolonged HRV persistence is found in a minority of children. Further studies are needed to evaluate whether this depends on host or virus characteristics.

    Susanna Esposito, MD1, Alessia Scala2, Beatrice Ascolese2, Laura Senatore2, Elisabetta Prada2, Cristina Daleno2, Monia Gambino2, Maria Vincenza Mastrolia2 and Nicola Principi2, (1)Pediatric Highly Intensive Care Unit, Univ Milan, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy, (2)Pediatric Highly Intensive Care Unit, Univ Milan, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy

    Disclosures:

    S. Esposito, None

    A. Scala, None

    B. Ascolese, None

    L. Senatore, None

    E. Prada, None

    C. Daleno, None

    M. Gambino, None

    M. V. Mastrolia, None

    N. Principi, None

    Findings in the abstracts are embargoed until 12:01 a.m. EDT, Oct. 8th with the exception of research findings presented at the IDWeek press conferences.

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