Characteristics of Human Rhinovirus Infection in Pediatric Age
Methods: From November 1, 2013, to March 31, 2014, a group of otherwise healthy children aged 1 to 18 months was studied. Parents were asked to collect every week a nasopharyngeal swab, to return to the Center for a monthly control visit and, when a febrile episode occurred, to complete a diary recording child’s clinical problems and medical prescriptions. RT-PCR was performed to identify HRV and positive samples were used for sequencing analysis and for reconstructing the phylogenetic tree.
Results: A total of 91 children was enrolled. HRV was identified in 516 swabs. All but 3 subjects had at least one swab positive in most of the cases without significant clinical problems. HRV types were identified in 392 samples (76.0%; HRV-A 151, 38.5%; -B 62, 15.8%; -C 179, 45.7%). Positivity for ≥4 consecutive samples was found in 38 (43.2%) children. Of these, only 12 had the same strain (A12 in 3 cases and one case each for A10, A76, A89, B14, B86, B103, C1, C25, C43).
Conclusion: This study indicates that in healthy children HRV tends to persist in the nasopharynx for a limited period of time and re-infection is the most common cause of repeated HRV identification in the same subject. Prolonged HRV persistence is found in a minority of children. Further studies are needed to evaluate whether this depends on host or virus characteristics.
B. Ascolese, None
L. Senatore, None
E. Prada, None
C. Daleno, None
M. Gambino, None
M. V. Mastrolia, None
N. Principi, None