Program Schedule

1676
Salivary Levels of Streptococcus mutans in Individuals with Temporomandibular Disorders

Session: Poster Abstract Session: Microbial and Host Factors
Saturday, October 11, 2014
Room: The Pennsylvania Convention Center: IDExpo Hall BC
Background:  Individuals with temporomandibular disorders (TMD) may experience higher levels of psychological stress and chronic pain, as well as some biochemical and immunological salivary changes; however, microbiological parameters related to dental caries have not yet been investigated in this population. 

Aim: To evaluate whether TMD patients present higher levels of Streptococcus mutans in saliva.

Methods:  Cross-sectional study which included 39 patients with TMD, selected according to the Fonseca’s questionnaire and the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD), and other 33 individuals with no TMD symptoms (control group). The volunteers were classified according to their caries risk in low, intermediate or high risk. Unstimulated saliva samples were collected from both TMD and the control group on the morning shift from 9 to 11am, at least one hour after the last meal. Aliquots of 100mL of each saliva sample were diluted, plated on Mitis Salivarius Agar containing Bacitracin, and then they were incubated (5% CO2, 37°C, 48h) in order to quantify S. mutans levels (CFU/mL); the colonies growth was confirmed by polymerase chain reaction (PCR).

Results:  When the Student t test was applied, there was no statistically significant difference between the TMD and the control group (p>0.05). 

Conclusion: Individuals with TMD did not show higher levels of S. mutans in saliva than the control group. Further studies investigating such a relationship should be carried out.

Leonardo Victor Galvão-Moreira1, Cláudia Monteiro De Andrade2, Jéssica Francisca Fernandes De Oliveira2, Patrícia De Maria Silva Figueiredo2 and Luciana Salles Branco-De-Almeida3, (1)Brock University, St. Catharines, ON, Brazil, (2)University Center of Maranhão, São Luís, Brazil, (3)Federal University of Maranhão, São Luís, Brazil

Disclosures:

L. V. Galvão-Moreira, FAPEMA: Grant Investigator, Research grant
FAPEMA: Grant Investigator, Research grant and Research support

C. Monteiro De Andrade, FAPEMA: Grant Investigator, Research grant

J. F. Fernandes De Oliveira, None

P. D. M. Silva Figueiredo, FAPEMA: Grant Investigator, Research support

L. S. Branco-De-Almeida, FAPEMA: Grant Investigator, Research support

Findings in the abstracts are embargoed until 12:01 a.m. EDT, Oct. 8th with the exception of research findings presented at the IDWeek press conferences.

Sponsoring Societies:

© 2014, idweek.org. All Rights Reserved.

Follow IDWeek