Salivary Levels of Streptococcus mutans in Individuals with Temporomandibular Disorders
Aim: To evaluate whether TMD patients present higher levels of Streptococcus mutans in saliva.
Methods: Cross-sectional study which included 39 patients with TMD, selected according to the Fonseca’s questionnaire and the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD), and other 33 individuals with no TMD symptoms (control group). The volunteers were classified according to their caries risk in low, intermediate or high risk. Unstimulated saliva samples were collected from both TMD and the control group on the morning shift from 9 to 11am, at least one hour after the last meal. Aliquots of 100mL of each saliva sample were diluted, plated on Mitis Salivarius Agar containing Bacitracin, and then they were incubated (5% CO2, 37°C, 48h) in order to quantify S. mutans levels (CFU/mL); the colonies growth was confirmed by polymerase chain reaction (PCR).
Results: When the Student t test was applied, there was no statistically significant difference between the TMD and the control group (p>0.05).
Conclusion: Individuals with TMD did not show higher levels of S. mutans in saliva than the control group. Further studies investigating such a relationship should be carried out.
L. V. Galvão-Moreira,
FAPEMA: Grant Investigator, Research grant
FAPEMA: Grant Investigator, Research grant and Research support
J. F. Fernandes De Oliveira, None
P. D. M. Silva Figueiredo, FAPEMA: Grant Investigator, Research support
L. S. Branco-De-Almeida, FAPEMA: Grant Investigator, Research support