Program Schedule

Prevalence and Molecular Epidemiology of ESBL producing E.coli and K.pneumoniae in Lebanese Medical Centers; Strong Correlation between Antibiotic Consumption and Resistance to Cephalosporins and Ciprofloxacin

Session: Poster Abstract Session: Antibiotic Stewardship
Thursday, October 9, 2014
Room: The Pennsylvania Convention Center: IDExpo Hall BC
  • E. coli poster final edit.pdf (271.7 kB)
  • Background: ESBL-producing Escherichia coli and Klebsiella pneumoniae have rapidly spread worldwide creating a severe threat. The development of resistance in these bacteria is multi-factorial; antibiotic consumption is a major factor. In view of both significant increase in ESBL production and lack of efficient control of antibiotic use in Lebanon (Middle East), we conducted a study to determine the epidemiology of ESBL-related genes in E.coli and K.pneumoniae, and analyzed the correlation of antibiotic use with bacterial resistance in 3 major medical centers.

    Methods: Three medical centers located in the north, south, and capital (Beirut) of Lebanon were chosen for this study. All ESBL producing E.coli and K.pneumoniae from inpatients were collected between 1/2/2012 and 1/2/2013. Only the 1st isolate per patient was included. Antibiotic consumption was expressed in DDD/100 bed days and Kirby-Bauer technique was used for antibiotic resistance testing. For the phenotypic detection of ESBL, double disk synergy test and E-test were performed. PCR and multiplex PCR were used for the detection of the genes blaTEM, blaSHV, blaCTX-M, and blaOXA. The identification of blaCTX-M15 was done by sequencing of the gene. The association between consumption and resistance was analyzed using Spearman correlation coefficient. Pulsed Field Gel Electrophoresis (PFGE) was used to determine the clonality of the different isolates.

    Results: A total of 1002 E.coli and 233 K.pneumoniae isolates were analyzed. 29.9% of E.coli strains and 31.1% of K.penumoniae were ESBL producers. Use of 3rd and/or 4th generation cephalosporins and resistance to these antibiotics were strongly correlated. In addition, the correlation factor of cephalosporin consumption versus susceptibility to ciprofloxacin in E.coli (Hospital 1) was as low as -0.899 and -0.886 in K. pneumoniae (Hospital 3). blaCTX-M was produced by 97% of E.coli and 96.7% of K.pneumoniae. Specifically, blaCTX-M15 was commonly found. 22.3% of E.coli and 37.4% of K.pneumoniae co-produced the 4 studied beta-lactamases.

    PFGE analysis demonstrated that there was no major clonal relationship among these ESBL producers.

    Conclusion: Our data show the urgent need for antimicrobial stewardship programs in the country to control the use of antibiotic and the spread of bacterial resistance.

    Ziad Daoud, Ph.D1, Elie Salem-Sokhn, PhD2, Jihad Irani, MD1, Katia Cheaito, MS3, Nathaline Haidar-Ahmad, MS3, Ghassan Matar, PhD3 and Shira Doron, MD, MS4, (1)Balamand University, Beirut, Lebanon, (2)University of Surrey, Surrey, United Kingdom, (3)American University of Beirut, Beirut, Lebanon, (4)Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center, Boston, MA


    Z. Daoud, Merck: Grant Investigator, Research grant

    E. Salem-Sokhn, None

    J. Irani, None

    K. Cheaito, None

    N. Haidar-Ahmad, None

    G. Matar, None

    S. Doron, Merck: Grant Investigator and Speaker's Bureau, Research grant and Speaker honorarium

    Findings in the abstracts are embargoed until 12:01 a.m. EDT, Oct. 8th with the exception of research findings presented at the IDWeek press conferences.

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