Comparison of two multiplex PCR techniques for the study of respiratory viruses in Mexican children with pneumonia
Methods: 310 nasal washings from children younger than 5 years old with clinical and/or radiological diagnosis of pneumonia were included. Nucleic acid was extracted, and amplified by two methods: xTAG RVP (Luminex Molecular Diagnostics, Toronto, Canada) and Anyplex II RV16 (Seegene, Seoul, Corea). The respiratory viruses detected were: RSV A and B, INF A and B, PIV1, 2, 3 and 4, AdV, MpV, CoV OC43, 229E and NL63, RV A/B/C, EV and HBoV1/2/3/4. The gold standard was a construct (the same result with both techniques, and those with discrepancies were sequenced). Virus frequencies, sensitivity, specificity, positive predictive value and negative predictive value were calculated.
Results: The viruses detected were in order of frequency: 43.1% RSV A, 22.9% RV/EV, 7.2% ADV, 6.5% INF A, 3.9% MpV, 2.9% HBoV, 2.6% PIV 3, 2.3% RSV B, 2.3% PIV 4, 2.3% CoV NL63, 1.9% PIV 1, 1.3% PIV 2, 0.9% INF B, 0.9% CoV OC43, 0.6% CoV 229E and 0.3% CoV HKUI.
Overall Anyplex II RV16 had a higher sensitivity (90% vs. 78.9%) and specificity (97.4% vs. 59.4%) compared to xTAG RVP. Anyplex II RV 16 was more sensitive in detecting AdV (100% vs. 52.2%), INF A (83.3% vs. 53.3%), RSV A (97.8% vs. 69.9%) and CoV OC45/HUK1 (100% vs. 50%) compared to xTAG RVP, respectively. xTAG RVP was more sensitive in detecting PIV (95% vs. 80%) , RSV B (80% vs. 71.4%), RV/EV (86.9% vs. 83.1%) and MpV (90% vs. 83.3%) compared to Anyplex II RV 16, respectively.
Conclusion: The 3 most frequent pathogens detected in children with pneumonia were RSV, RV and PIV (1/2/3/4). Overall, Anyplex II RV16 had a higher sensitivity and specificity than xTAG RVP for the diagnosis of multiple respiratory viruses. Although, xTAX RVP is more sensitive to detect PIV, RSV B, RV and MpV.
A. P. Gonzalez-Rodriguez,
C. M. Alpuche Aranda, None
I. Lopez Martinez, None
T. Hernandez Andrade, None
J. Gaitan Meza, None
D. Noyola, None
A. Villaseñor Sierra, None
G. Martinez Aguilar, None
L. F. Perez Gonzalez, None
O. A. Newton Sanchez, None
V. Firo Reyes, None
C. N. Del Rio Almendarez, None
J. I. Santos Preciado, None
R. M. Wong Chew, Seegene: Grant Investigator, Research grant
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