Program Schedule

Comparison of two multiplex PCR techniques for the study of respiratory viruses in Mexican children with pneumonia

Session: Poster Abstract Session: Diagnostic Microbiology: Viruses/Fungal/AFB/Parasitic
Saturday, October 11, 2014
Room: The Pennsylvania Convention Center: IDExpo Hall BC
  • Philadelphia RV FINAL.pdf (1.3 MB)
  • Background: Respiratory tract infections are the main cause of morbidity and mortality worldwide. Diagnostic methods have evolved through time and now there are techniques available to detect multiple viruses in one sample. The aims of the study were to identify respiratory viruses in nasal washings from children younger than 5 years old admitted with pneumonia at 6 hospitals from 6 cities in Mexico and to perform a diagnostic test evaluation between 2 multiplex PCR techniques for the detection of respiratory viruses.

    Methods: 310 nasal washings from children younger than 5 years old with clinical and/or radiological diagnosis of pneumonia were included. Nucleic acid was extracted, and amplified by two methods: xTAG RVP (Luminex Molecular Diagnostics, Toronto, Canada) and Anyplex II RV16 (Seegene, Seoul, Corea).  The respiratory viruses detected were: RSV A and B, INF A and B, PIV1, 2, 3 and 4, AdV, MpV, CoV OC43, 229E and NL63, RV A/B/C, EV and HBoV1/2/3/4. The gold standard was a construct (the same result with both techniques, and those with discrepancies were sequenced). Virus frequencies, sensitivity, specificity, positive predictive value and negative predictive value were calculated.

    Results: The viruses detected were in order of frequency: 43.1% RSV A, 22.9% RV/EV, 7.2%  ADV, 6.5% INF A, 3.9% MpV, 2.9% HBoV, 2.6% PIV 3, 2.3% RSV B, 2.3% PIV 4, 2.3% CoV NL63, 1.9% PIV 1, 1.3% PIV 2, 0.9% INF B, 0.9% CoV OC43, 0.6% CoV 229E and 0.3% CoV HKUI.

    Overall Anyplex II RV16 had a higher sensitivity (90% vs. 78.9%) and specificity (97.4% vs. 59.4%) compared to xTAG RVP. Anyplex II RV 16 was more sensitive in detecting AdV (100% vs. 52.2%), INF A (83.3% vs. 53.3%), RSV A (97.8% vs. 69.9%) and CoV OC45/HUK1 (100% vs. 50%) compared to xTAG RVP, respectively. xTAG RVP was more sensitive in detecting PIV (95% vs. 80%) , RSV B (80% vs. 71.4%), RV/EV (86.9% vs. 83.1%) and MpV (90% vs. 83.3%) compared to Anyplex II RV 16, respectively.

    Conclusion: The 3 most frequent pathogens detected in children with pneumonia were RSV, RV and PIV (1/2/3/4). Overall, Anyplex II RV16 had a higher sensitivity and specificity than xTAG RVP for the diagnosis of multiple respiratory viruses.  Although, xTAX RVP is more sensitive to detect PIV, RSV B, RV and MpV.

    Alejandra Pamela Gonzalez-Rodriguez, BSc1, Miguel Leonardo Garcia Leon, MSc2, Celia Mercedes Alpuche Aranda, MD DSc3, Irma Lopez Martinez, MSc4, Teresa Hernandez Andrade, BSc4, Jesus Gaitan Meza, MD5, Daniel Noyola6, Alberto Villaseñor Sierra, MD DSc7, Gerardo Martinez Aguilar, MD, MSc.8, Luis Fernando Perez Gonzalez, MD9, Oscar Alberto Newton Sanchez, MD, MSc.10, Veronica Firo Reyes, MD11, Carlos Nicolas Del Rio Almendarez, MD12, Jose Ignacio Santos Preciado, MD2 and Rosa Maria Wong Chew, MD DSc13, (1)Experimental Medicine, Universidad Nacional Autonoma de México, Mexico City, Mexico, (2)Experimental Medicine Department, Universidad Nacional Autonoma de Mexico, DF, Mexico, (3)Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico, (4)Instituto Nacional de Diagnostico y referencia epidemiologica, DF, Mexico, (5)Infectious Diseases, Nuevo Hospital Civil de Guadalajara, Guadalajara, Mexico, (6)Facultad de Medicina, Universidad Autonoma de San Luis Potosi, San Luis Potosi, Mexico, (7)CIBO. CMNO, IMSS Guadalajara, Guadalajara, Mexico, (8)IMSS Durango, Durango, Mexico, (9)Pediatric Infectious Diseases, Hospital Central Ignacio Morones Prieto, San Luis Potosi, Mexico, (10)Pediatrics, Hospital Regional Universitario de los Servicios de Salud del Estado de Colima, Colima, Colima, Mexico, (11)Pediatrics, Hospital General de Mexico, DF, Mexico, (12)Infectious Diseases, Hospital Pediatrico de Coyoacan, DF, Mexico, (13)Experimental Medicine Department, Universidad Nacional Autonoma De Mexico, DF, Mexico


    A. P. Gonzalez-Rodriguez, None

    M. L. Garcia Leon, None

    C. M. Alpuche Aranda, None

    I. Lopez Martinez, None

    T. Hernandez Andrade, None

    J. Gaitan Meza, None

    D. Noyola, None

    A. Villaseñor Sierra, None

    G. Martinez Aguilar, None

    L. F. Perez Gonzalez, None

    O. A. Newton Sanchez, None

    V. Firo Reyes, None

    C. N. Del Rio Almendarez, None

    J. I. Santos Preciado, None

    R. M. Wong Chew, Seegene: Grant Investigator, Research grant

    Findings in the abstracts are embargoed until 12:01 a.m. EDT, Oct. 8th with the exception of research findings presented at the IDWeek press conferences.

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