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128
A whole-blood transcriptional signature predicts parasite control and protection from malaria fever during natural Plasmodium falciparum infection

Session: Oral Abstract Session: Immune Response to Microbial Infection
Thursday, October 9, 2014: 11:45 AM
Room: The Pennsylvania Convention Center: 111-AB
Background: Understanding the host response to natural malaria infection can aid in the rational design of a highly effective vaccine. Antibody-mediated immunity that confers non-sterile protection from febrile malaria is acquired gradually through repeated P. falciparum blood-stage infections. However, the nature of cellular immune responses at the onset of clinically apparent versus clinically silent blood-stage infections in children remains to be elucidated.

Methods: In a prospective study in Mali, we collected whole-blood RNA, PBMCs and plasma from healthy, uninfected children aged 6-11 years (n=78) before the 6-month malaria season and from the same children during their first P. falciparum infection of the ensuing season. First infections were detected retrospectively through bi-weekly active surveillance by PCR. We used RNA-seq to compare whole-blood transcriptomes of children whose clinically silent infections never progressed to fever (immune, n=20), children whose infections progressed to fever within 2-14 days (late fever, n=32) and children who were febrile at the time of infection (early fever, n=26).

Results: Baseline transcription profiles before the malaria season distinguished children whose future P. falciparum infections either progressed to fever or not. Transcription profiles induced by the first-detected P. falciparum infection of the season revealed upregulation of innate, pro-inflammatory responses in immune children but not in late fever children, despite both groups having similar levels of parasitemia and the clinical absence of fever initially. In addition, this early upregulation of innate, pro-inflammatory responses was associated with slower subsequent parasite growth rates in vivo. In ongoing work, we are correlating transcription profiles with P. falciparum-specific antibody profiles before, during and after the first infection using P. falciparum protein microarrays.

Conclusion: Molecular and cellular signatures that predict protection from clinical malaria and biologically relevant outcomes are yielding novel insights into the mechanisms underlying naturally acquired immunity to malaria. The resulting datasets may inform the development of interventions that prevent or mitigate malaria disease.

Tuan M. Tran, M.D., Ph.D.1, Aissata Ongoiba, M.D.2, Marcus Jones, Ph.D.3, Jeff Skinner, M.S.1, Shanping Li, M.S.1, Safiatou Doumbo, M.D.2, Didier Doumtabe, Pharm.D.2, Younoussou Kone, M.D.2, Aboudramane Bathily, M.D.2, Jules Sangala, M.D.2, Ogobara K. Doumbo, M.D., Ph.D.2, Pratap Venepally, Ph.D.3, Kassoum Kayentao, M.D., M.P.H.2, Boubacar Traore, Pharm.D., Ph.D.2, Ewen F. Kirkness, Ph.D.3 and Peter D. Crompton, M.D., M.P.H.1, (1)Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, (2)Mali International Center of Excellence in Research, Bamako, Mali, (3)Genomic Medicine Group, J. Craig Venter Institute, Rockville, MD

Disclosures:

T. M. Tran, None

A. Ongoiba, None

M. Jones, None

J. Skinner, None

S. Li, None

S. Doumbo, None

D. Doumtabe, None

Y. Kone, None

A. Bathily, None

J. Sangala, None

O. K. Doumbo, None

P. Venepally, None

K. Kayentao, None

B. Traore, None

E. F. Kirkness, None

P. D. Crompton, None

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