Longterm carriage of Ciprofloxacin-Resistant E. Coli Isolates among Nursing Home (NH) Residents
Methods: We used REP-PCR to type 86 ciprofloxacin-resistant E. coli isolates collected during monthly surveillance of 21 individuals with medical devices over a 1 year period in a single NH. Isolates were collected from multiple body sites at each time point and screened for antibiotic susceptibility against ciprofloxacin. REP-PCR amplification was performed on all isolates using (GTG)5-primers; isolates from the same individual were run on the same gel. To check if isolates were shared among individuals, a representative strain from each type was sampled from each individual and run on a single gel. Strains with the identical banding pattern were considered the same.
Results: Most isolates were from the rectum (49%) and the groin (24%). Nine unique REP-PCR-types were observed; 4 individuals (19%) carried at least two genetically unique strains. The median duration of carriage for a single strain was 28 days (range, 1-338 days). One REP -PCR type was found in 7 residents and two among 4 residents; 3 REP-PCR types were found on only one resident suggesting substantial transmission among residents.
Colonized NH residents tended to carry the same ciprofloxacin-resistant E. coli strain at multiple body sites and for long periods. 90% of REP -PCR types were found in multiple/at least two individuals suggesting that the risk of transmission of resistant strains of E. coli is high in NH.
U. Srinivasan, None
L. Mody, None
S. Mcnamara, University of Michigan - Geriatrics: Employee, Salary
AHRQ: Collaborator, Salary and staff member on AHRQ grants and contracts
NIH : Collaborator, Salary and staff member on NIH grants and contracts of Dr. Mody
B. Lansing, None
B. Foxman, None
See more of: Poster Abstract Session