Eradication of Medically Important Multidrug Resistant Bacteria and Fungi Using PurpleSun Inc. Multivector UV Technology
Methods: Three isolates of each following resistant bacterial pathogens and pathogenic fungi were used in the study: methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), ESBL Escherichia coli (EC), carbapenemase-resistant Klebsiella pneumoniae (KPC), multidrug-resistant Pseudomonas aeruginosa (PA), Acinetobacter baumannii (AB), Clostridium difficile (CD), Candida albicans (CA), C. glabrata (CG), C. parapsilosis (CP), C. krusei (CK), Aspergillus fumigatus (AF), Fusarium solani (FS), and Scedosporium apiospermum (SA). Plates containing two different sizes of inoculum 1-5 ´ 103-104 or 1-5 ´ 101-102were exposed to UV energy at different times (s) and locations in the UV field, according to the design of the study.
Results: Full clearance of the organism from the plates was achieved at 15 s of exposer for MRSA and PA. Clearance of VRE, EC, CG, CP, CK, FS, and SA was achieved after 30 s of exposure. KPC, AB, CA, and CD demonstrated no growth after 60 s of exposure to the UVC. The longest time, 120 s, was required to clear AF. The rate of clearance of organisms from the plates didn’t depend on inoculum size.
Conclusion: The study demonstrated that UVC disinfection unit eradicates tested resistant bacteria and pathogenic fungi effectively and rapidly. Inoculum size did not affect rate of kill of tested organisms. This study provides foundation for the future testing of the UVC disinfection unit in the clinical environment.
A. N. Schuetz, None
K. Kennedy-Norris, None
J. H. Powers, None
S. L. Dalton, None
E. Petraityte, None
K. A. Hussain, None
M. L. Kyaw, None
T. J. Walsh, None