Antibodies to conformational epitopes interfere with T20 fusion inhibition
Methods: Our lab has previously isolated the antibody gene sequences of a number of trimer-specific antibodies. These bind to four seemingly conformational epitopes. We established an assay to assess functional interference with T20 fusion inhibition. We then screened a panel of control antibodies and trimer-specific and neutralizing antibodies in this assay.
Results: Two of our trimer specific antibodies that were epitope mapped to gp41 inhibit fusion of t20 in this assay. We established a production and functional screening method for antibodies that target structural epitopes to HIV and report novel findings that our trimer-specific gp41 targeting Abs interfere with T20 fusion inhibition.
Conclusion: Antibodies targeting these epitopes have implications in both potential therapy strategies and vaccine development.
M. Hicar, None