1878. Low pertussis toxin antibody levels in maternal and cord blood samples
Session: Poster Abstract Session: Vaccines: Pertussis
Saturday, October 10, 2015
Room: Poster Hall
Posters
  • zafer kurugol_ IDSA.pdf (527.9 kB)
  • Background: Despite the presence of effective immunization programs with high vaccination coverage, pertussis continues to cause significant mortality and morbidity in many countries. The highest incidence of complications and deaths occur in infants younger than 3 months who have not received their complete primary vaccination series. The young infants depend on maternal antibodies for protection until their primary immunization series against pertussis are complete. The aim of the study was to determine anti-pertussis antibody levels (anti-PT and anti-FHA) in cord and maternal blood and to evaluate the efficacy of transplasental antibody transfer.  

    Methods: Paired maternal and umbilical cord blood samples were collected at the time of delivery. Antibody titers to pertussis toxin (anti-PT) and filamentous hemagglutinin (anti-FHA) in each paired sample of maternal and cord blood were measured by in-house enzyme linked immunosorbent assay (ELISA). Geometric mean concentrations (GMCs) of pertussis antibodies and cord:maternal GMC ratios were calculated. Antibody levels of <10 EU/ml was accepted as non-immune, and ≥100 EU/ml was accepted as possible acute/recent infection.

    Results: A total of 251 paired maternal delivery and cord blood samples were investigated in the study. The mean maternal age was 28.2 years, and the mean gestational age was 39.2 weeks. GMCs of IgG to PT and to FHA in maternal delivery serum were 4.12 and 9.89 EU/ml, respectively; GMCs of IgG to PT and FHA in cord blood were 5.49 and 12.73 EU/ml, respectively. GMCs of IgG in cord blood were 1.3 times higher than GMCs in maternal delivery serum. Placental transfer of pertussis antibodies was 133% for PT and 131% for FHA. Anti-PT levels (≥10 EU/ml) were found only in 25.1% of maternal samples and 34.7% of cord blood samples. The prevalence of recent pertussis infection in pregnant women was 3.6%.

    Conclusion: Anti-pertussis antibody concentrations were low in pregnant women in Turkey. Despite antibody levels were 1.3 fold higher in cord blood than in maternal blood, cord antibody concentrations are too low, possibly placing infants at risk until their primary vaccinations against pertussis are complete. These results support the rationale for maternal pertussis immunization in order to prevent mothers and infants against pertussis.

    Zafer Kurugol Sr., Prof1, Ebru Turkoglu, MD2, Nilay Coplu, Ass Prof3, Esra Ozer, Ass. Prof2 and Cemile Sonmez, MD3, (1)Pediatrics, Ege University, Izmir, Turkey, (2)Pediatrics, Tepecik Hospital, Izmir, Turkey, (3)Microbiology Reference Laboratories, Turkish Public Health Institution, Ankara, Turkey

    Disclosures:

    Z. Kurugol Sr., None

    E. Turkoglu, None

    N. Coplu, None

    E. Ozer, None

    C. Sonmez, None

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