Methods: Retrospective, descriptive, observational study analyzing 113 blood samples collected from October 2014 through April 2015. Positive blood samples identified by traditional culture method Phoenix™, then analyzed using MALDI-TOF within 48 hours of confirmed positive gram-positive (GP), gram-negative (GN), anaerobe or yeast organism. Reference used to analyze samples is integrated into the BioTyper software version 220.127.116.11 and compared to a research use only database. Each isolate given a spectral score, evaluated using software, and confirmed correct identification if score value > 2.0 at species level or > 1.7 but < 2.0 at genus level.
Results: Total of 113 (48GP, 40GN, 14 anaerobe, and 11 yeast) analyzed using MALDI-TOF. Thirty-six (90%) GN, twenty-nine (60%) GP , nine (64%) anaerobe, and three (27%) yeast organisms were correctly identified using MALDI-TOF. Time to identification per organism using MALDI-TOF was 30 minutes.
Conclusion: Achieved 90% concordance with GN, 60% with GP, 64% with anaerobes, and 27% with yeast organisms. Annual cost savings using MALDI-TOF for initial gram-positive identification is approximately $57,000 and dramatically shortens time to identification. This has a direct positive impact on patient care.
H. Sikand, None