Methods: Samples were screened by nested PCR using two sets of primers. Positive samples were sequenced and subjected to phylogenetic analysis. Patients with RTI were screened during a four years period from April 2010 to March 2014.
Results: From four hundred fifty nine hospitalized children and adult patients with RTI, 17 patients (3.7%) were diagnosed with WUV infections. Genomic WUV DNA of three strains were sequenced and phylogenetic trees were constructed. Two of the circulating WUV strains belong to the type Ia and the third one belongs to the type IIIb. This study enabled us to identify the circulating WUV genotypes among patients with viral RTI in Kuwait.
Conclusion: These finding allow us to develop an understanding of the circulating WUV genotypes among these patients. Precise classification and typing procedures will be important in the future for classifying WUV isolates and investigating what role these genetic groups play in human biology and disease.
W. Chehadeh, None